摘要
目的探究lncRNA DUXAP8在子宫内膜癌发生发展中的作用。方法使用癌症基因组图谱(TCGA)数据库分析lncRNA DUXAP8在子宫内膜癌组织中的表达水平以及DUXAP8与患者预后的关系,qRT-PCR检测DUXAP8在子宫内膜癌细胞系中的表达情况。通过转染构建了DUXAP8过表达或敲低的HEC-1A细胞系。CCK-8、克隆形成、Transwell、划痕实验和流式细胞术实验分别检测不同转染处理后子宫内膜癌细胞的增殖、侵袭、迁移、周期和凋亡情况,Western blot实验检测上皮细胞间质转化(EMT)相关蛋白的表达水平。结果DUXAP8在子宫内膜癌中显著上调,DUXAP8高表达的子宫内膜癌患者预后相对较差。抑制DUXAP8的表达可以阻碍肿瘤细胞的增殖、侵袭、迁移和EMT进程,细胞周期将阻滞于G0/G1期,并能诱导细胞凋亡的发生;过表达DUXAP8后肿瘤细胞的增殖、侵袭、迁移和EMT进程显著增强,细胞周期阻滞于S期,细胞凋亡水平显著降低。结论DUXAP8在子宫内膜癌中起促癌基因的作用,有望成为子宫内膜癌治疗的新靶标。
Objective The aim of this study was to explore the role of lncRNA DUXAP8 in the occurrence and development of endometrial cancer(EC).Methods The Cancer Genome Atlas(TCGA)database was used to analyze the expression level of lncRNA DUXAP8 in EC tissues and the association between DUXAP8 and prognosis of patients.qRT-PCR was used to detect the expression of DUXAP8 in EC cells lines.HEC-1A cell lines with DUXAP8 overexpressed or knockdown were constructed by transfection.CCK-8,colony forming,transwell,scratch healing assay,and flow cytometry were used to detect the abilities of proliferation,invasion,migration,cell cycle,and cell apoptosis in endometrial cancer cells after different treatments,and Western blot was used to detect the levels of epithelial-mesenchymal transition(EMT)-related protein.Results DUXAP8 was significantly upregulated in endometrial cancer,and endometrial cancer patients with high DUXAP8 expression had a relatively poor prognosis.Inhibition of DUXAP8 hinder cell proliferation,invasion,migration and EMT process,cell cycle arrested at the G0/G1 phase,and induced apoptosis.While overexpression DUXAP8 enhanced the proliferation,invasion,migration and EMT process of tumor cells,cell cycle arrested at the S phase,and apoptosis level significantly reduced.Conclusion These results suggested that DUXAP8 functions regard as a pro-oncogene in endometrial cancer and may be a new target for EC therapy.
作者
夏建洪
周立庆
严研
马婷婷
苏欣宇
XIA Jianhong;ZHOU Liqing;YAN Yan;MA Tingting;SU Xinyu(Department of Radiotherapy,The Affiliated Huai′an Hospital of Xuzhou Medical University and The Second People′s Hospital of Huai′an,Huai′an 223002,China)
出处
《实用肿瘤学杂志》
CAS
2023年第2期137-143,共7页
Practical Oncology Journal
基金
淮安市自然科学研究计划项目(编号:HAB202029)。
关键词
子宫内膜癌
DUXAP8
增殖
凋亡
Endometrial cancer
Double homeobox A pseudogene 8
Proliferation
Apoptosis
作者简介
夏建洪,男,(1973-),学士,副主任医师,从事肿瘤学方向的研究;通信作者:周立庆,E-mail:adkfhe_3815@163.com。
