摘要
【目的】明确抗菌药物盐酸多西环素和恩诺沙星对织锦巴非蛤肠道菌群的影响,为织锦巴非蛤健康养殖提供参考依据。【方法】分别以10.0 mg/L盐酸多西环素和10.0 mg/L恩诺沙星浸泡织锦巴非蛤1 d后,采用PacBio高通量全长16S rRNA基因测序技术对其肠道微生物的多样性和组成进行比较分析。【结果】抗菌药物处理组织锦巴非蛤肠道菌群的Alpha多样性指数与对照组(CK)无显著差异(P>0.05);在门分类水平,织锦巴非蛤肠道微生物的优势菌群为变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes),同时弯曲杆菌门(Campylobacter)在2种抗菌药物处理组均显著降低(P<0.05,下同);在属分类水平,CK的主要优势菌属为Malaciobacter、粉色海生菌属(Roseimarinus)、Poseidonibacter和克雷伯菌属(Klebsiella),盐酸多西环素处理组的主要优势菌属为交替单胞菌属(Alteromonas)、Rubritalea和科尔韦尔氏菌属(Colwellia),恩诺沙星处理组的主要优势菌属为极地杆菌属(Polaribacter)、Aurantivirga和假浅黄色杆菌(Pseudofulvibacter),3个处理组织锦巴非蛤肠道菌群中的主要优势菌属均不同;2种抗菌药物处理可使织锦巴非蛤肠道中Malaciobacter、粉色海生菌、Poseidonibacter和Halarcobacter的相对丰度显著降低。【结论】10.0 mg/L盐酸多西环素和10.0 mg/L恩诺沙星曝露对织锦巴非蛤肠道菌群的丰度和多样性无显著影响,但使其肠道菌群结构和组成发生改变,同时,织锦巴非蛤肠道中某些有害菌的相对丰度降低。
【Objective】To provide a reference basis for the healthy breeding of Paphia amabilis,the effects of doxycycline hydrochloride and enrofloxacin on the intestinal microbial community of P.amabilis were studied.【Method】The diversity and composition of intestinal microbial community of P.amabilis were analyzed by PacBio high-throughput fulllength 16S rRNA gene sequencing technology after being exposed with doxycycline hydrochloride and enrofloxacin respectively at a concentration of 10.0 mg/L for 1 d.【Result】Results indicated that there was no significant difference inα-diversity index of intestinal microbial community between antibiotics treatment group and control group(CK)(P>0.05).At the phylum level,the dominant intestinal microbial community in P.amabilis were Proteobacteria and Bacteroidetes.In addition,Campylobacter decreased significantly in the two antibiotic treatment groups(P<0.05,the same below).At the genus level,the main dominant bacteria of CK were Malacioactor,Roseimarinus,Poseidonibacter and Klebsiella,and in the doxycycline hydrochloride treatment group were Alteromonas,Rubritalea and Colwellia,while in the enrofloxacin treatment group were Polaribacter,Aurantivirga and Pseudofulvibacter.The dominant bacteria in the intestinal microbial community of the three treatments were different;antibiotics exposure decreased significantly the relative abundances of Malaciobacter,Roseimarinus,Poseidonibacter and Halarcobacter.【Conclusion】The study shows that there is no significant change on the abundance and diversity of intestinal microbial community under the short-term exposure to 10.0 mg/L doxycycline hydrochloride and 10.0 mg/L enrofloxacin,but it changes the structure and composition of intestinal microbial community.Meanwhile,the treatment of antibiotics reduce the relative abundance of some harmful bacteria in the intestine of P.amabilis.
作者
熊向英
曾梦清
陈瑞芳
XIONG Xiang-ying;ZENG Meng-qing;CHEN Rui-fang(Guangxi Academy of Marine Science,Guangxi Academy of Sciences/Guangxi Key Laboratory of Marine Environmental Science,Nanning,Guangxi 530007,China;Guangxi Institute of Oceanology Co.,Ltd./Guangxi Engineering Technology Research Center of Breeding of New Mariculture Varieties,Beihai,Guangxi 536000,China;Beibu Gulf Marine Industry Research Institute,Fangchenggang,Guangxi 538000,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2023年第1期273-280,共8页
Journal of Southern Agriculture
基金
广西千亿元产业科技攻关成果产业化引导项目(桂科转1599002-13)
广西自然科学基金项目(2020GXNSFBA159011)
广西重点研发计划项目(桂科AB18221112)
广西科学院基本科研业务费项目(2019YJJ1004)。
作者简介
第一作者:熊向英(1984-),https://orcid.org/0000-0002-3969-4172,副研究员,主要从事水产健康养殖研究工作,E-mail:xiangying 841027@126.com;通讯作者:陈瑞芳(1978-),https://orcid.org/0000-0003-3953-0052,副研究员,主要从事贝类育苗与健康养殖研究工作,E-mail:ttrfchen@foxmail.com。
