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鹿茸菇母种培养基条件优化及其菌丝显微观察 被引量:1

Condition Optimization of the Mother Culture Medium for Lyophyllum decastesand the Microscopic Observation of Its Mycelium
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摘要 为进一步了解鹿茸菇的生物学特性,筛选其适宜的母种培养基配方,设置了不同碳源、氮源、pH和温度条件的筛选试验,观测鹿茸菇菌丝生长情况和菌丝显微结构。结果表明:鹿茸菇母种培养基菌丝生长最适碳源为淀粉,最适氮源为硝酸钠,最适pH值为7.0,最适菌丝生长温度为25℃,鹿茸菇菌丝为双核细胞,有锁状联合,其细胞较长,不同生长时期隔膜明显度不同。 In order to further understand the biological characteristics of Lyophyllum decastes and select the appropriate formula of mother culture medium,the screening tests of different carbon sources,nitrogen sources,pH and temperature conditions were set up to observe the mycelial growth and microstructure of Lyophyllum decastes.The results showed that the optimum carbon source was starch,the optimum nitrogen source was sodium nitrate,the optimum pH was 7.0,and the optimum temperature for the mycelial growth was 25℃.The mycelium of Lyophyllum decastes was a binuclear cell with clamp connection.Its cells were longer,and the visibility of cell membrane was different at different growth stages.
作者 陈华 黄彩翔 郭月仙 林怡 王义祥 刘朋虎 CHEN Hua;HUANG Cai-xiang;GUO Yue-xian;LIN Yi;WANG Yi-xiang;LIU Peng-hu(Agricultural Ecology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;Fujian Key Laboratory of Agricultural Ecological Process in Hilly Areas with Red Soil,Fuzhou,Fujian 350003,China;National Research Center of Juncao Engineering Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Henglv Biotechnology Co.,Ltd.,Ningde,Fujian 355300,China)
出处 《福建农业科技》 CAS 2023年第2期22-27,共6页 Fujian Agricultural Science and Technology
基金 福建省科技计划星火项目(2020S0065)。
关键词 鹿茸菇 母种培养基 细胞核 显微观察 Lyophyllum decastes Mother culture medium Cellular nuclear Microscopic observation
作者简介 陈华,男,1976年生,副研究员,主要从事农业生态学研究;通信作者:刘朋虎,男,1982年生,博士,研究员,主要从事食用菌育种与循环农业技术研究(E-mail:phliu1982@163.com)。
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