摘要
为明确洛阳烟区烟草白粉病的病原菌及其寄主植物种类,通过形态学观察和分子生物学方法鉴定了从洛阳烟区采集的烟草白粉病样品的病原菌,并采用致病性测定与分子生物学分析相结合的方法,检测其在洛阳烟区的寄主范围。结果表明:(1)洛阳烟区烟草白粉病病原菌为烟草高氏白粉菌(Golovinomyces tabaci);(2)除烟草外,采集的烟田周边22种感染白粉病植物的病原菌均不为烟草高氏白粉菌(G.tabaci)且这些病原菌对烟草均不具有致病性;(3)采集的烟草白粉病样品的病原菌对烟田周边22种健康植物中车前草和豌豆具有致病性,分子生物学鉴定结果进一步证实了车前草和豌豆为洛阳烟区烟草白粉病病原菌的寄主植物。
In order to identify the pathogen and host plant species of tobacco powdery mildew in Luoyang tobacco-growing area,the tobacco powdery mildew pathogens collected from Luoyang tobacco-growing area were identified by morphological observation and molecular biological determination.The host range in Luoyang tobacco-growing area was determined by combining pathogenicity evaluation with molecular biological analysis.The results showed that:1)The pathogen of tobacco powdery mildew in Luoyang tobacco area was Golovinomyces tabaci.2)In addition to tobacco,22 kinds of powdery mildew pathogens collected from plants infected with powdery mildew around tobacco fields were not G.tabaci and those pathogens had no pathogenicity to tobacco.3)The collected tobacco powdery mildew pathogen was pathogenic to plantain and pea,which were two plants in 22 common plants around tobacco fields.The results of molecular biological identification further confirmed that plantain and pea were the host plants of tobacco powdery mildew pathogen in Luoyang tobacco-growing area.
作者
李佳
王树和
周俊学
宋正熊
康业斌
LI Jia;WANG Shuhe;ZHOU Junxue;SONG Zhengxiong;KANG Yebin(College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471023,Henan,China;Luoyang Branch of Henan Provincial Tobacco Company,Luoyang 471023,Henan,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2023年第4期33-40,共8页
Tobacco Science & Technology
基金
河南省烟草公司洛阳市公司科技项目“洛阳烟区烟叶白粉病流行规律及防治技术研究”(LYKJ202109)。
关键词
烟草
烟草白粉病
烟草高氏白粉菌
形态学鉴定
分子生物学鉴定
寄主植物
Nicotiana tabacum L
Tobacco powdery mildew
Golovinomyces tabaci
Morphological identification
Molecular biological identification
Host plant
作者简介
第一作者:李佳(1998-),女,在读硕士研究生,从事植物病理学研究。E-mail:2480020902@qq.com;通信作者:康业斌(1964-),男,博士,教授,博士生导师,从事植物病理学研究。E-mail:kangyb999@163.com。
