摘要
探讨β-葡萄糖苷酶在甜菊糖苷生物转化中的作用。通过添加不同浓度β-葡萄糖苷酶水解乙醇超声提取的甜菊糖苷,探究最适酶浓度、温度与pH条件,用HPLC法测定甜菊糖苷、莱鲍迪苷A与斯替夫苷的含量,以确定β-葡萄糖苷酶水解作用效果。结果表明,在pH 7.0时添加600 U/g的β-葡萄糖苷酶,于乙醇溶液中超声(44.7 Hz、60℃、1 h)、水解(40℃、2 h)提取的甜菊糖苷,用HPLC测定甜叶菊茎秆中甜菊糖苷含量为0.167 mg/g,莱鲍迪苷A含量为0.125 mg/g,斯替夫苷含量为0.022 mg/g;叶片中甜菊糖苷含量为0.184 mg/g,莱鲍迪苷A含量为0.153 mg/g,斯替夫苷含量为0.035 mg/g,叶片甜菊糖苷含量高于茎秆;与未经β-葡萄糖苷酶处理对照相比,甜叶菊茎秆中甜菊糖苷含量提高28.7%,莱孢迪苷A含量提高23.7%,斯替夫苷含量提高15.8%;叶片中甜菊糖苷含量提高19.9%,莱孢迪苷A含量提高25.7%,斯替夫苷含量提高21.9%。β-葡萄糖苷酶可有效水解甜叶菊茎秆与叶片中的糖苷类物质,提高甜菊糖苷得率,茎秆中的甜菊糖苷也得以被提取利用。
To investigate the role ofβ-glucosidase in the biotransformation of stevia,by adding different concentration ofβ-glycosidase enzymes hydrolysis ethanol ultrasonic extraction of stevia glycoside,the optimum enzyme concentration,temperature and pH condition was studied,and stevia sugar was determined by HPLC method,rebaudioside-A(RA)and stevioside(St)concentration,to determine theβ-glycosidase enzymes hydrolysis effect.The results showed that at pH7.0,600 U/gβ-glucosidase was added,and the stevioside extracted by ultrasonic ethanol was hydrolyzed at 40℃(60℃,44.7 Hz,2 h).The content of stevioside in stevia stem was 0.167 mg/g,and the content of reb A was 0.125 mg/g.The content of stevenside was 0.022 mg/g;The content of stevioside in leaves was 0.184 mg/g,the content of reboudiside A was 0.153 mg/g,the content of stevioside in leaves was 0.035 mg/g,and the content of stevioside in leaves was higher than that in stems.Compared with the control without β-glucosidase treatment,the content of stevioside in the stem of Stevia was increased by 28.7%,the content of lysine was increased by 23.7%,and the content of stevioside was increased by 15.8%.The content of stevioside in leaves was increased by 19.9%,the content of lysine was increased by 25.7%,and the content of stevioside was increased by 21.9%.Theβ-glucosidase could effectively hydrolyze the glycosides in the stem and leaves of Stevia,which could improve the yield of stevia,and the stevia in the stem could also be extracted and utilized.
作者
童红梅
宋巧
张敏
郭敏
何丹
TONG Hongmei;SONG Qiao;ZHANG Min;GUO Min;HE Dan(Gansu Medical College,Pingliang 744000)
出处
《食品工业》
CAS
2023年第3期181-184,共4页
The Food Industry
基金
甘肃医学院青年基金(甜叶菊β-葡萄糖苷酶糖苷酶的分离纯化及酶学特性的研究(GYSF2021-02))
甘肃医学院校级(β-葡萄糖苷酶对甜菊糖苷生物转化作用与调控机制研究GY-2022ZK006)
平凉市科技局重点研发计划(陇东地区甜叶菊产业化试验引种技术研究PLKJJH2021003)。
作者简介
通信作者:宋巧。
