摘要
Spinal cord injury(SCI)is a serious clinical disease.Due to the deformability and fragility of the spinal cord,overly rigid hydrogels cannot be used to treat SCI.Hence,we used TPA and Laponite to develop a hydrogel with shear-thinning ability.This hydrogel exhibits good deformation,allowing it to match the physical properties of the spinal cord;additionally,this hydrogel scavenges ROS well,allowing it to inhibit the lipid peroxidation caused by ferroptosis.According to the in vivo studies,the TPA@Laponite hydrogel could synergistically inhibit ferroptosis by improving vascular function and regulating iron metabolism.In addition,dental pulp stem cells(DPSCs)were introduced into the TPA@Laponite hydrogel to regulate the ratios of excitatory and inhibitory synapses.It was shown that this combination biomaterial effectively reduced muscle spasms and promoted recovery from SCI.
基金
This study was partly funded by grants from the National Natural Science Funding of China(82172424,82271629)
Outstanding Youth Fund of Zhejiang Province(LR22H060002)
Zhejiang Medical and Health Science and Technology Plan Project(2022RC210,2021KY212)
Wenzhou Basic Science Research Plan Project(Y20210045)
CAMS Innovation Fund for Medical Sciences(2019-I2M-5-028).
作者简介
Yibo Ying,contributed equally to this work;Zhiyang Huang,contributed equally to this work;Yurong Tu,contributed equally to this work;Qiuji Wu,contributed equally to this work;Corresponding author:Sipin Zhu,Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,Zhejiang,China.E-mail addresses:sipinzhu@163.com;Corresponding author:Zhouguang Wang,School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou,Zhejiang,325000,China.E-mail addresses:wzhouguang@gmail.com;Corresponding author:Qingsong Ye,Center of Regenerative Medicine,Renmin Hospital of Wuhan University,Wuhan,460030,Hubei,China.E-mail addresses:qingsongye@hotmail.com。
