摘要
睾丸支持细胞(Sertoli cells,SCs)和精原干细胞(spermatogonial stem cells,SSCs)发生相应变化才能维持正常精子发生。因此,探讨生精上皮发育中两者的关系对深入了解精子发生有重要意义。基于前期基础,分别取青春期前(出生后5,10 d)、青春期(15,20 d)、近性成熟(25,30 d)及性成熟后(35,40,50,70 d)的昆明雄性小鼠,采用本室已建立的曲细精管漂浮免疫荧光染色全铺片法,测量了小鼠各阶段曲细精索/管的直径;以GATA4为SCs标记、胶质细胞源神经营养因子家族受体α-1(GFRα-1)为SSCs分子标记,显示生精上皮中的SCs和SSCs并分析其数量变化。结果显示,随日龄增加其曲细精索/管直径显著增大,特别是在性成熟前;5 d小鼠的GATA4+SCs数量最少,随后快速上升,15 d时达到最高且显著高于其他各阶段(P<0.05),之后呈波动下降趋势且趋于相对稳定;GFRα-1+SSCs数量也随日龄增大而增多,15 d时达到最高且显著高于其他各阶段(P<0.01),随后在20~30 d有所下降,35 d后急剧下降且处于相对稳定状态。提示小鼠SCs与SSCs数量之间基本呈正相关,SCs在维持SSCs的增殖、分化及正常精子发生方面有重要作用。本研究对探讨其他动物和人的精子发生机理有一定借鉴意义。
The testicular Sertoli cells(SCs)and spermatogonial stem cells(SSCs)undergo corresponding changes in order to maintain normal spermatogenesis during the development.Therefore,exploring the relationship between SCs and SSCs during the development of the seminiferous epithelium is of great significance for profound understanding of the spermatogenesis.Based on our previous work,Kunming white male mice at the pre-puberty(5,10 d),puberty(15,20 d),near sexual maturity(25,30 d)and post-sexual maturity(35,40,50,70 d)were used as the experimental animals.The free-floating immunofluorescence staining of the seminiferous tubules(whole mount method)established in our laboratory was used to measure the diameters of mouse seminiferous cords/tubules at different developmental stages,by using GATA4 as the SC marker and glial cell-derived neurotrophic factor family receptor alpha-1(GFRα-1)as the SSC marker.The results showed that the diameters of the seminiferous cords/tubules increased significantly along with age,especially before the sexual maturity.Mice at 5 d have the fewest number of GATA4+SCs,and then it increased rapidly,reaching the highest at 15 d and significantly higher than those of other developmental stage(P<0.05).The SC number showed a fluctuating downward trend and tended to be relatively stable after 20d.The number of GFRα-1+SSCs also increased along with the age,and reached the highest at 15dand significantly higher than other stages(P<0.01).Then SSC numbers decreased during 20-30d,and sharply decreased after 35dwith a relatively stable state afterwards.These results indicated that there was a positive correlation between the number of SCs and SSCs in mice,and SCs played an important role in maintaining the proliferation,differentiation of SSCs and normal spermatogenesis.The present study provides certain reference for understanding the spermatogenesis mechanism of other animals and humans.
作者
张博洋
车冠宇
杨蕊
朱春玲
陈斓歆
赵彦森
张洪毅
潘英树
唐博
张学明
ZHANG Boyang;CHE Guanyu;YANG Rui;ZHU Chunling;CHEN Lanxin;ZHAO Yansen;ZHANG Hongyi;PAN Yingshu;TANG Bo;ZHANG Xueming(College ofVeterinary Medicine,Jilin University,Changchun 130062,China;Beijing SeekGene BioSciences Co.,Ltd.,Beijing 102206,China;College 0f Animal Science,Jilin University,Changchun 130062,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2023年第3期598-602,609,共6页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31872434,32172803)。
作者简介
张博洋(1998-),男,硕士研究生;共同第1作者:车冠宇(1988-),女,硕士;通讯作者:张学明,E-mail:zhangxuem@jlu.edu.cn。
