摘要
炭疽菌是核桃主要病害核桃炭疽病的病原,目前对核桃炭疽菌携带的病毒种类及病毒基因组序列信息了解较少。利用宏病毒组测序技术,对分离自我国3个不同省份的25株核桃炭疽菌所携带的病毒基因序列进行发掘、鉴定和分类。经同源比对分析,获得22种病毒基因组序列,其中19种为新病毒。在22种病毒中,有21种为正单链RNA病毒,1种为dsRNA病毒。正单链RNA分别隶属于裸露病毒科(Narnaviridae)、线粒体病毒科(Mitoviridae)和葡萄孢欧尔密病毒科(Botourmiaviridae),而dsRNA属于Alternaviridae病毒科。RT-PCR验证结果表明22种病毒都能在核桃炭疽菌株中被检测到,且25株炭疽菌的病毒携带率为100%,每个菌株都至少被1-11种病毒侵染。研究结果丰富了炭疽菌属所携带的病毒基因组信息,为后续深入分析炭疽菌真菌病毒的多样性和分子特性奠定基础。
Colletotrichum spp.are the pathogens of walnut anthracnose,a major disease of walnuts,but little has been known about the types and their genomic sequence information of viruses carried by phytopathogenic fungus Colletotrichum spp.of walnut.In this study,25 strains isolated from walnut fruits suffering walnut anthracnose in three different provinces of China were mined,identified and classified via metatranscriptomic approach.After homologous alignment analysis,22 kinds of viral genome sequences were obtained,19 of them were novel.Among the 22 mycoviruses,21 were positive single-stranded RNA viruses and one was dsRNA virus.The positive single-stranded RNAs belonged to family Narnaviridae,Mitoviridae and Botourmiaviridae,respectively,while the dsRNA belonged to Alternaviridae family.The RT-PCR validation results showed that all 22 mycoviruses were detected in our Colletotrichum spp.strains,and the virus carriage rate of 25 strains was 100%,with each strain being infected by at least 1 to 11 viruses.The results enriched the genomic information of viruses carried by Colletotrichum spp.and laid the foundation for the subsequent in-depth analysis of the diversity and molecular characteristics of mycoviruses from Colletotrichum.
作者
徐小文
李金仓
海都
查玉平
宋菲
王义勋
XU Xiao-wen;LI Jin-cang;HAI Du;ZHA Yu-ping;SONG Fei;WANG Yi-xun(Hubei Academy of Forestry,Wuhan 430075;Huazhong Agricultural University,Wuhan 430070)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第3期278-289,共12页
Biotechnology Bulletin
基金
湖北省林业科学研究院基金项目(2021YJJ01)。
关键词
真菌病毒
核桃炭疽菌
宏病毒组测序
多样性
mycovirus
pathogen of walnut anthracnose(Colletotrichum spp.)
meta-transcriptomic sequencing
diversity
作者简介
徐小文,博士,助理研究员,研究方向:森林病害防治,E-mail:xuxiaowen222@126.com;通讯作者:王义勋,博士,副研究员,研究方向:森林病虫害防治,E-mail:594580124@qq.com。
