摘要
目的:探讨miR-155是否通过调控Wnt/β-catenin通路影响滋养细胞的生物学行为,参与子痫前期(PE)的发生。方法:收集20例PE孕妇(PE组)及20例正常孕妇(Normal组)的胎盘组织,qRT-PCR检测组织中miR-155表达,qRT-PCR及Western blot检测β-catenin表达。HTR-8/SVneo细胞分别转染miR-155 mimic、miR-155 inhibitor及miR-155 NC,qRT-PCR检测其转染效率及β-catenin mRNA表达,Western blot检测β-catenin及Wnt3a蛋白表达,CCK-8、Transwell、流式细胞术分别检测细胞的增殖、迁移及凋亡。转染miR-155 inhibitor后,用β-catenin抑制剂处理细胞,检测相关蛋白表达及细胞的增殖、迁移和凋亡。结果:PE组胎盘组织中miR-155表达较Normal组升高(P<0.001),β-catenin表达降低(P<0.01)。过表达miR-155后,β-catenin及Wnt3a表达、细胞增殖、迁移能力均下降(P<0.01),凋亡上升(P<0.001);抑制miR-155表达后,β-catenin及Wnt3a表达、细胞增殖、迁移能力均升高,凋亡减少(P<0.01)。β-catenin抑制剂处理细胞可逆转miR-155低表达对相关蛋白表达及细胞生物学行为的影响(P<0.05)。结论:miR-155在PE胎盘组织中高表达,其可能通过负向调控Wnt/β-catenin信号通路进而抑制滋养细胞的增殖、迁移,促进其凋亡,参与PE发生。
Objective:To investigate whether miR-155 affects the biological behavior of trophoblast cells by regulating Wnt/β-catenin pathway and participates in the occurrence of preeclampsia.Methods:The placental tissues of 20 cases of preeclamptic pregnant women(PE group)and 20 cases of normal pregnant women(normal group)were collected.qRT-PCR was used to detect the expression of miR-155 in tissues,qRT-PCR and Western blot were used to detect the expression ofβ-catenin.HTR-8/SVneo cells were transfected with miR-155 mimic,miR-155 inhibitor and miR-155 NC.qRT-PCR was used to detect the transfection efficiency and the expression ofβ-catenin mRNA.Western blot was used to detect the expression ofβ-catenin and Wnt3a protein.CCK-8,transwell and flow cytometry were used to detect the proliferation,migration and apoptosis of the cells.After transfection with miR-155 inhibitor,cells were treated withβ-catenin inhibitor,and then the expression of related proteins,cell proliferation,migration and apoptosis were detected.Result:Expression of miR-155 in placental tissue in PE group was higher than normal group(P<0.001)and expression ofβ-catenin decreased(P<0.01).After overexpression of miR-155,the expression ofβ-catenin and Wnt3a,cell proliferation and migration were decreased(P<0.01),while apoptosis was increased(P<0.001).After inhibiting the expression of miR-155,the expressions ofβ-catenin and Wnt3a,cell proliferation and migration ability were increased,and apoptosis was decreased(P<0.01).Treatment withβ-catenin inhibitor could reverse the effect of miR-155 low expression on the expression of related proteins and cell biological behavior(P<0.05).Conclusions:miR-155 is highly expressed in PE placenta,which may be involved in the occurrence of preeclampsia by negatively regulating Wnt/β-catenin signaling pathway to inhibit the proliferation and migration of trophoblast cells and promote apoptosis.
作者
曾莹
岳巾晶
郭晓珮
董越
彭瑞
罗晓华
Zeng Ying;Yue Jinjing;Guo Xiaopei(Department of Obstetrics and Gynecology,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052)
出处
《现代妇产科进展》
北大核心
2023年第3期177-181,共5页
Progress in Obstetrics and Gynecology
基金
河南省科技攻关项目(No:212102310473)。
作者简介
通信作者:罗晓华,Email:Luoxiaohua620@163.com;第一作者:曾莹(1997-),女,郑州大学第三附属医院住院医生。主要研究方向:病理妊娠、围产医学。
