摘要
Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.
基金
supported by the National Natural Science Foundation of China(31922072)
Natural Science Foundation of Shandong Province(ZR2020JQ15)
Project of Shandong Province Higher Educational Science and Technology Program(2019KJF012)
Taishan Scholar Project of Shandong Province(tsqn201812020)
China Agriculture Research System(CARS-48)
Qingdao Science and Technology Demonstration and Guidance Project for Benefiting the People(20-3-4-28-nsh)。
作者简介
Corresponding authors at:Xiangzhao Mao,College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China.E-mail address:xzhmao@ouc.edu.cn;Corresponding authors:Zhen Liu,atliuzhenyq@ouc.edu.cn。
