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基于LC-MS/MS技术研究稻草替代部分玉米青贮对奶牛血浆代谢产物的影响 被引量:1

LC-MS/MS-based study on effect of rice straw instead of partial corn silage on plasma metabolites of dairy cows
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摘要 为探讨稻草替代部分玉米青贮后对奶牛的血浆代谢物的影响,本文利用LC-MS/MS物质分离鉴定技术,结合多元统计方法对稻草替代部分玉米青贮前后的代谢产物进行分析。结果表明:稻草替代部分玉米青贮后,两组奶牛血浆中共检测到41种差异代谢物。与对照组相比,试验组奶牛血浆中肾上腺素、β-羟丁酸、烟酰胺、胆酸等22个代谢物上调,谷胱甘肽、皮质醇、皮质酮等19个代谢物下调。由此可见:稻草替代部分玉米青贮后,奶牛脂肪动员增强,抗应激能力下降。研究结果为稻草替代玉米青贮在奶牛日粮中的应用提供参考,为稻草资源的开发利用提供新思路。 In order to investigate the effect of rice straw replacing part of corn silage on plasma metabolites of dairy cows,LC-MS/MS material separation and identification technology combined with multivariate statistical method was used in this paper to analyze the metabolites of rice straw before and after replacing corn silage.The results showed that after rice straw replaced part of corn silage,a total of 41 differential metabolites were detected in the plasma of the two groups.Twenty-two metabolites including epinephrine,β-hydroxybutyric acid,nicotinamide and cholic acid were up-regulated,and 19 metabolites such as glutathione,cortisol and corticosterone were down-regulated in the experimental group.It could be seen that replacing part of corn silage with rice straw enhanced the fat mobilization of dairy cows and reduced the ability to resist stress.The results provided a reference for the application of rice straw instead of corn silage in dairy cow diet,and provided a new idea for the development and utilization of rice straw resources.
作者 李娅楠 冶文兴 朱相德 陈林 徐晓锋 张力莉 LI Yanan;YE Wenxing;ZHU Xiangde;CHEN Lin;XU Xiaofeng;ZHANG Lili(College of Agriculture,Ningxia University,Yinchuan 750021,China)
机构地区 宁夏大学农学院
出处 《浙江农业学报》 CSCD 北大核心 2023年第2期266-274,共9页 Acta Agriculturae Zhejiangensis
基金 国家自然科学基金(31660675)。
关键词 稻草 玉米青贮 奶牛 血浆 代谢物 rice straw corn silage dairy cow plasma metabolite
作者简介 李娅楠(1997-),女,河南平顶山人,硕士研究生,研究方向为反刍动物营养。E-mail:18837172663@163.com;通信作者:张力莉,E-mail:zhanglilinx@126.com。
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