摘要
为了探究不同比例的α-生育酚及α-生育三烯酚抗氧化能力的大小,共开展2个试验。试验一∶设置4个不同比例的α-生育酚及α-生育三烯酚,分别为α-生育酚(TE组)、α-生育酚∶α-生育三烯酚=6.5∶1(TT1组)、α-生育酚∶α-生育三烯酚=2.75∶1(TT2组)、α-生育酚∶α-生育三烯酚=1.5∶1(TT3组),生育酚及生育三烯酚之和均为100μmol/L,测定其羟自由基清除率、超氧阴离子清除率。试验二∶选用与试验一相同比例的α-生育酚及α-生育三烯酚,与猪小肠上皮细胞(IPEC-J2)共培养6 h后加入敌草快(Diquat,DQ)处理4 h,用CCK-8法测定细胞活力,用流式细胞仪测定细胞内活性氧含量。试验结果表明∶不同比例的α-生育酚与α-生育三烯酚对羟自由基的清除率存在差异,其中TT3组羟自由基清除率显著高于TE组(P<0.05),其余各组间无显著性差异;超氧阴离子清除率也受α-生育酚与α-生育三烯酚比例的影响,TT1组超氧阴离子清除率显著高于TE组(P<0.05),TT2、TT3组极显著高于TE组(P<0.01)。细胞培养试验表明∶敌草快处理后极显著降低了细胞活力(P<0.001),TT2预处理组细胞活力显著高于敌草快处理组(P<0.05),TT3预处理组细胞活力极显著(P<0.01)高于敌草快处理组,TT3组抵抗敌草快氧化应激的效果最佳;不同比例的α-生育酚与α-生育三烯酚对细胞内活性氧的含量也有显著的影响,与Ctrl组相比,加入敌草快后,各试验组细胞内活性氧含量极显著上升(P<0.01),与DQ组相比,TE+DQ、TT1+DQ、TT2+DQ组细胞内活性氧含量均显著下降(P<0.05),TT3+DQ组细胞内活性氧含量极显著下降(P<0.01),与TE+DQ组相比,TT2+DQ、TT3+DQ组细胞内活性氧含量显著下降(P<0.05)。因此∶不同比例的α-生育酚及α-生育三烯酚的抗氧化能力不同,其中α-生育酚∶α-生育三烯酚=1.5∶1时,其羟自由基清除率、超氧阴离子清除率最高;对IPEC-J2细胞的预保护作用最好,抗氧化能力最佳。
In order to investigate the antioxidant capacity ofα-tocopherol andα-tocotrienol in different proportions,two experiments were conducted.In experiment 1,four different proportions ofα-tocopherol andα-tocotrienol were set up,respectively:α-tocopherol(TE group),α-tocopherol∶α-tocotrienol=6.5∶1(TT1 group),α-tocopherol∶α-tocotrienol=2.75∶1(TT2 group),α-tocopherol∶α-tocotrienol=1.5∶1(TT3 group),the sum of tocopherol and tocotrienol were 100μmol/L,and the scavenging rates of hydroxyl radical and superoxide anion were measured.In experiment 2,α-tocopherol andα-tocotrienol,the same proportions as in experiment 1,were co-cultured with porcine small intestine epithelial cells(IPEC-J2)for 6h and treated with diquat(DQ)for 4h.Cell viability was determined by CCK-8 method and intracellular reactive oxygen species content was determined by flow cytometry.The results showed that the hydroxyl radical scavenging rates ofα-tocopherol andα-tocotrienol were different,and the hydroxyl radical scavenging rates of TT3 group were significantly higher than those of TE group(P<0.05),but there was no significant difference among other groups.The superoxide anion clearance rate was also affected by the ratio ofα-tocopherol toα-tocotrienol.The superoxide anion clearance rate of TT1 group was significantly higher than that of TE group(P<0.05),and that of TT2 and TT3 groups was significantly higher than that of TE group(P<0.01).The cell culture test showed that the cell viability was significantly decreased(P<0.001)after treatment with diquat,the cell vitality of the TT2 group was significantly higher than that of DQ group(P<0.05),and the cell vitality of the TT3 group was significantly higher than that of DQ group(P<0.001).TT3 group had the best resistance to the oxidative stress of diquat.Different proportions ofα-tocopherol andα-tocotrienol also had significant effects on the content of intracellular reactive oxygen species.Compared with control group,the content of intracellular reactive oxygen species in experimental groups was significantly increased after the addition of diquat(P<0.01).Compared with DQ group,The intracellular reactive oxygen content in TE+DQ,TT1+DQ and TT2+DQ groups was significantly decreased(P<0.05),and the intracellular reactive oxygen content in TT3+DQ group was extremely significantly decreased(P<0.01).Compared with TE+DQ group,the intracellular reactive oxygen content in TT2+DQ and TT3+DQ groups was significantly decreased(P<0.05).The antioxidant capacity ofα-tocopherol andα-tocopherol with different proportions were different.Whenα-tocopherol∶α-tocopherol=1.5∶1,the hydroxyl radical scavenging rate and superoxide anion scavenging rate were the highest.It had the best preprotection effect on IPEC-J2 cells and the best antioxidant capacity.
作者
任颖
赵嘉楠
孙海辉
京芳华
代志凯
廖英俊
黄辉其
王艳青
马立保
REN Ying;ZHAO Jia-nan;SUN Hai-hui;JING Fang-hua;DAI Zhi-kai;LIAO Ying-jun;HUANG Hui-qi;WANG Yan-qing;MA Li-bao(College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Yichun Big Turtle Life Science Co.LTD.Yichun 336000,China)
出处
《粮食与饲料工业》
CAS
2023年第1期39-46,共8页
Cereal & Feed Industry
基金
中央引导地方科技发展资金:猪用α-生育三烯酚饲料添加剂新产品的开发及作用机制研究(赣科发计字【2022】112号)。
作者简介
任颖(1999-),女,硕士,研究方向为动物营养与饲料学;通讯作者:马立保(1965-),男,副教授,研究方向为猪的营养与代谢调控;通讯作者:王艳青(1969-),女,副教授,研究方向为反刍动物的营养需要及矿物质营养的研究。
