摘要
浮游与附生细菌群落在湿地氮污染物去除及其水质净化方面具有重要作用,然而与湿地土壤细菌群落相比,它们的群落动态还远未被了解.以榆古桥人工湿地水体浮游细菌群落和常见沉水植物苦草(Vallisneria natans)叶表附生细菌群落为研究对象,通过高通量测序的方法对浮游和附生细菌群落的组成、结构与功能多样性进行了比较分析.结果表明,浮游与附生细菌群落的组成具有显著差异,相比浮游细菌群落,附生细菌群落中存在更多的异养细菌和反硝化细菌.浮游细菌群落的α多样性在3个采样点间存在显著差异,而附生细菌群落的α多样性没有显著差异;整体来看,附生细菌群落的OTU指数与Shannon指数显著高于浮游细菌群落且二者存在明显的空间异质性.RDA分析表明DO、IC、TP、NH_(4)^(+)和TOC对浮游和附生细菌群落的结构变化均具有重要影响,但对浮游细菌群落的影响更大.共现性网络分析表明附生细菌群落有更多的生态位分化,网络更加稳定,对外界干扰的抵抗力也更强.FAPROTAX功能预测结果分析表明,附生细菌群落的氮循环特别是反硝化作用显著大于浮游细菌群落.研究结果揭示了浮游和附生细菌群落多样性维持的驱动机制,可以为人工湿地建设中发掘和利用水体浮游细菌和附生细菌群落资源以提高净化水质效率提供科学依据.
Planktonic and epiphytic bacterial communities play an important role in wetland nitrogen pollutant removal and water purification,yet their community dynamics are far from understood compared with those of the wetland soil bacterial community.Taking the planktonic bacterial community in the Yuguqiao constructed wetland and the epiphytic bacterial community on the leaf surface of the common submerged plant Vallisneria natans as the research objects,the composition,structure,and functional diversity of planktonic and epiphytic bacterial communities were analyzed using high-throughput sequencing.The results showed that the compositions of the planktonic and epiphytic bacterial communities were significantly different,with more heterotrophic and denitrifying bacteria present in the epiphytic bacterial community than in the planktonic bacterial community.Theαdiversity of the planktonic bacterial community was significantly different among the three sampling sites but not in the epiphytic bacterial community.In general,the OTU index and Shannon index of the epiphytic bacterial community were significantly higher than those of the planktonic bacterial community,and they had obvious spatial heterogeneity.RDA analysis showed that DO,IC,TP,NH_(4)^(+),and TOC had important effects on the structural changes of both planktonic and epiphytic bacterial communities but had a greater impact on planktonic bacterial communities.Co-occurrence network analysis showed that the epiphytic bacterial community had more niche differentiation,a more stable network,and stronger resistance to external disturbance.The results of FAPROTAX functional prediction analysis showed that the nitrogen cycling,especially denitrification of the epiphytic bacterial community,was significantly greater than that of the planktonic bacterial community.The results of this study revealed the driving mechanism for maintaining the diversity of planktonic and epiphytic bacterial communities,which can provide a scientific basis for excavating and utilizing planktonic and epiphytic bacterial community resources in the construction of constructed wetlands to improve the efficiency of water purification.
作者
张梅婷
刘晋仙
苏嘉贺
柴宝峰
ZHANG Mei-ting;LIU Jin-xian;SU Jia-he;CHAI Bao-feng(Institute of Loess Plateau,Shanxi University,Taiyuan 030006,China;Shanxi Yellow River Laboratory,Taiyuan 030006,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2023年第1期252-261,共10页
Environmental Science
基金
国家自然科学基金项目(31801962)
山西省基础研究项目(201901D211129,201901D211457)。
关键词
附生细菌
浮游细菌
多样性
苦草
湿地
epiphytic bacterial
planktonic bacterial
diversity
Vallisneria natans
wetland
作者简介
张梅婷(1997-),女,硕士研究生,主要研究方向为水生微生物生态学,E-mail:894362914@qq.com;通信作者:刘晋仙,E-mail:liujinxian@sxu.edu.cn。
