摘要
目的:分析基质金属蛋白酶-2(MMP-2)、肌球蛋白轻链激酶(MLCK)和硫酸基转移酶1A3(SULT1A3)在子宫肌瘤组织中的表达及与子宫肌瘤发生发展的可能关系。方法:收集2019年1月-2021年6月在本院行手术切除治疗的子宫肌瘤患者60例子宫肌瘤组织和正常子宫组织,分别采用RT-PCR技术和免疫组化法测定MMP-2、MLCK、SULT1A3的mRNA及蛋白表达,分析各指标表达与子宫肌瘤发生的相关性。结果:子宫肌瘤组织MMP-2(1.61±0.23)、MLCK(11.87±1.60)的mRNA相对表达量均高于正常子宫组织(1.05±0.09、6.14±1.02),SULT1A3的mRNA相对表达量(0.69±0.11)低于正常子宫组织(1.65±0.44);子宫肌瘤组织中MMP-2蛋白阳性率(68.3%)、MLCK蛋白阳性率(76.7%)均高于正常子宫组织(25.0%、26.7%),子宫肌瘤组织SULT1A3蛋白阳性率(61.7%)低于正常子宫组织(100.0%)(均P<0.05)。mRNA表达,MMP-2与MLCK呈正相关,MMP-2、MLCK与SULT1A3均呈负相关性;蛋白表达,MMP-2与MLCK蛋白表达呈正相关,MMP-2、MLCK与SULT1A3蛋白表达均呈负相关性(均P<0.05)。结论:子宫肌瘤组织中MMP-2、MLCK呈高表达,SULT1A3呈低表达,且各指标具有相关性,这种异常表达可能与子宫肌瘤的发生有关。
Objective: To analyze the expressions of matrix metalloproteinase-2(MMP-2), myosin light chain kinase(MLCK) and sulfotransferases 1A3(SULT1A3) in uterine leiomyoma tissue of patients, and to study their correlation with the uterine leiomyoma occurrence. Methods: The uterine leiomyoma tissues and normal uterine tissues of 60 patients who had undergone surgical resection of uterine leiomyoma from January 2019 to June 2021 were collected. The mRNA expression levels of MMP-2, MLCK, and SULT1A3 were detected by RT-PCR, and the protein expressions of MMP-2, MLCK, and SULT1A3 were detected by immunohistochemistry. The correlation between the expressions of mRNA and protein of MMP-2, MLCK, and SULT1A3 and the occurrence of uterine leiomyoma was analyzed. Results: The mRNA relative expression levels of MMP-2(1.61±0.23) and MLCK(11.87±1.60) in uterine leiomyoma tissues were significantly higher than those(1.05±0.09 and 6.14±1.02) in normal uterine tissues. The mRNA relative expression level of SULT1A3(0.69±0.11) in uterine leiomyoma tissues was significantly lower than that(1.65±0.44) in normal uterine tissue. The positive rates of MMP-2 protein(68.3%) and MLCK protein(76.7%) in uterine leiomyoma tissues were significantly higher than those(25.0% and 26.7%) in normal uterine tissue, and the positive rates of SULT1A3 protein(61.7%) in uterine leiomyoma tissues was significantly were lower than that(100.0%) in normal uterine tissue(all P<0.05). The mRNA expression of MMP-2 was positive correlation with the mRNA expression of MLCK. The mRNA expressions of MMP-2 and MLCK were negative correlation with the mRNA expression of SULT1A3. The protein expression of MMP-2 was positive correlation with the protein expression of MLCK. The protein expressions of MMP-2and MLCK were negative correlation with the protein expression of SULT1A3(all P<0.05).Conclusion:The expression levels of MMP-2and MLCK in the uterine leiomyoma tissue are high,and the expression level of SULT1A3 in the uterine leiomyoma tissue is low,and which have correlation each other.It is suggesting that the abnormal expressions of MMP-2,MLCK,and SULT1A3may be closely related to the occurrence of uterine leiomyoma.
作者
王建华
龚钿
张继旺
WANG Jianhua;GONG Dian;ZHANG Jiwang(Meishan City People’s Hospital,Meishan,Sichuan Province,620000;The Second People’s Hospital of Chongqing)
出处
《中国计划生育学杂志》
2023年第2期429-433,共5页
Chinese Journal of Family Planning
