摘要
以脱脂紫背天葵粉为原料,依次采用碱法提取、活性炭脱色、木瓜蛋白酶脱蛋白制备紫背天葵多糖,并研究了其化学组成、溶解性、膨润度及乳化性。结果显示,紫背天葵多糖含有质量分数为83.41%的总糖、5.18%灰分和1.28%蛋白质;该多糖由10种单糖组成,包括岩藻糖、核糖、葡萄糖醛酸、鼠李糖、甘露糖、阿拉伯糖、半乳糖、木糖、半乳糖醛酸与葡萄糖,其摩尔比为0.30:2.45:5.39:5.43:6.67:19.20:30.11:205.29:315.51:3173.30;它含有α-吡喃环,具有较好的分子均一性。紫背天葵多糖在20℃水中的溶解度高达93.13%,在体积分数为30%~70%的乙醇溶液中的溶解度和膨润度分别为28.07%~80.13%和38.71%~71.49%,静置20 h后其乳化值为78.78%。紫背天葵多糖具有良好的水溶性、醇溶性、膨润度与乳化性。
Defatted G.bicolor powder was used as raw material,G.bicolor polysaccharide was obtained by alkaline extraction,activated carbon decolorization and papain deproteinization in turn,and its chemical composition,solubility,swelling degree and emulsification capacity were studied.The results showed that the polysaccharide contained 83.41%(w/w)total sugar,5.18%ash and 1.28%proteins,and it was composed of 10 kinds of monosaccharides including fucose,ribose,glucuronic acid,rhamnose,mannose,arabinose,galactose,xylose,galacturonic acid,glucose in the molar ratio of 0.30:2.45:5.39:5.43:6.67:19.20:30.11:205.29:315.51:3173.30.The polysaccharide containedα-pyran ring and had good molecular homogeneity.The solubility of the polysaccharide in water at 20℃was up to 93.13%,and its solubility and swelling degree in 30%~70%(v/v)ethanol solutions were 28.07%~80.13%and 38.71%~71.49%respectively.The emulsification value of the polysaccharide was 78.78%after 20 h of standing.G.bicolor polysaccharide shows good water solubility,solubility and swelling degree in ethanol and emulsification.
作者
张雪儿
金野
杜沁岭
徐文
胡佳颖
贾冬英
ZHANG Xue’er;JIN Ye;DU Qinling;XU Wen;HU Jiaying;JIA Dongying(College of Biomass Science&Engineering,Sichuan University,Chengdu 610065,China;Qionglai Tianfu Modern Seed Industry Park Management Committee,Qionglai 611530,China)
出处
《食品科技》
CAS
北大核心
2022年第11期179-184,共6页
Food Science and Technology
基金
中央高校基本科研业务费专项基金项目(2021CDZG-19)。
关键词
紫背天葵
多糖
化学组成
物理特性
Gynura bicolor
polysaccharide
chemical composition
physical property
作者简介
张雪儿(2000-),女,安徽六安人,硕士研究生,研究方向为食物资源开发利用;通信作者:贾冬英。