摘要
为筛选金花菜和紫花苜蓿的耐盐基因,本研究以盐胁迫下的金花菜(JHC)和紫花苜蓿(ZHMX)试管苗为试验材料进行转录组分析。结果表明:金花菜组和紫花苜蓿组差异基因数为26722,金花菜组vs紫花苜蓿组有15850个基因下调,有10872个基因上调。金花菜组和紫花苜蓿组差异基因KEGG富集显著的Pathways有核糖体、光合作用天线蛋白、糖酵解/糖异生、光合作用、苯丙烷生物合成等。金花菜组耐盐基因有碱性亮氨酸拉链43、NAC转录因子47、ABC转运A家族成员7、晚期胚胎发生丰富蛋白2、乙烯反应转录因子ERF110等基因;紫花苜蓿组耐盐基因有WRKY转录因子、蛋白TIFY 8、转录因子MYB13、核运输因子2A、低温盐响应蛋白等基因。本试验结果可为了解金花菜和紫花苜蓿的耐盐分子机制及选育金花菜和紫花苜蓿耐盐新品种提供理论依据。
In order to screen the salt tolerance genes of Medicago polymorpha L.and Medicago sativa L.,the plantlets of Medicago polymorpha L.(JHC)and Medicago sativa L.(ZHMX)under salt stress were used for transcriptome analysis.The results showed that the number of differential genes between JHC group and ZHMX group was 26722.15850 genes were down regulated and 10872 genes were up regulated in JHC group vs ZHMX group.Pathways with significant enrichment of KEGG gene in JHC group and ZHMX group included ribosome,photosynthesis antenna protein,glycolysis/gluconeogenesis,photosynthesis,phenylpropanoid biosynthesis,etc.Salt tolerance genes in JHC group included basic leucine zipper 43,NAC transcription factor 47,ABC transporter A family member 7,late embryogenesis abundant protein 2,ethylene responsive transcription factor ERF110,etc.The salt tolerance genes in ZHMX group included WRKY transcription factor,protein TIFY 8,transcription factor MYB13,nuclear transport factor 2A,low temperature and salt responsive protein,etc.The results of this experiment could provide a theoretical basis for understanding the molecular mechanism of salt tolerance of Medicago polymorpha L.and Medicago sativa L.and breeding new salt tolerant varieties of Medicago polymorpha L.and Medicago sativa L..
作者
洪森荣
朱盈盈
李紫莹
胡明艳
欧阳克蕙
HONG Senrong;ZHU Yingying;LI Ziying;HU Mingyan;OUYANG Kehui(College of Life Sciences,Shangrao Normal University,Shangrao,Jiangxi 334001;Shangrao Agricultural Technology Innovation Research Institute,Shangrao,Jiangxi 334001;Key Laboratory of Protection and Utilization of Medicinal and Edible Plant Resources in Shangrao City,Shangrao,Jiangxi 334001;Shangrao Key Laboratory of Germplasm Conservation and Utilization of Potato and Taro Crops,Shangrao,Jiangxi 334001;College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045)
出处
《中国农学通报》
2023年第3期111-118,共8页
Chinese Agricultural Science Bulletin
基金
江西省现代农业产业技术体系建设专项(JXARS-13-赣东站)
上饶市科技局平台载体建设项目(2020I001)
上饶师范学院2022年大学生创新创业训练计划项目(202210416011)。
关键词
盐胁迫
金花菜
紫花苜蓿
试管苗
转录组分析
耐盐基因
salt stress
Medicago polymorpha L.
Medicago sativa L.
plantlet
transcriptome analysis
salt tolerance gene
作者简介
第一作者:洪森荣,男,1974年出生,江西永新人,教授,硕士,主要从事植物生物技术方面的研究。E-mail:hongsenrong@163.com;通讯作者:欧阳克蕙,女,1974年出生,江西万安人,教授,博士,主要从事牧草资源开发与利用和南方草地生态的研究。通信地址:330045江西农业大学动物科学技术学院,Tel:0791-83813503,E-mail:ouyangkehui@sina.com。
