摘要
目的 探讨外周血单核细胞DNA甲基化转移酶1(DNMT1)及血清白介素-6(IL-6)在糖尿病肾脏病(DKD)中的表达及临床意义。方法 选取150例DKD患者(DKD组)及50例同期健康体检者(对照组)。比较2组IL-6、DNMT1、总胆固醇、三酰甘油、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血肌酐、空腹血糖、肾小球滤过率估计值(e GFR)、24 h尿白蛋白排泄率(24 h UAER)的差异。Pearson相关分析IL-6、DNMT1与其他临床指标的相关性。多因素Logistic回归分析DKD发生的影响因素。受试者工作特征(ROC)曲线分析24 h UAER、IL-6、DNMT1及联合检测对DKD的诊断价值。结果 与对照组比较,DKD组的空腹血糖、IL-6、DNMT1、血肌酐、24 h UAER、LDL-C、糖化血红蛋白水平升高,而eGFR、HDL-C水平降低(均P<0.05)。DKD组的IL-6与DNMT1呈正相关(r=0.560,P<0.05);且IL-6、DNMT1与24 h UAER呈正相关(r分别为0.551和0.570,P<0.05)。24 h UAER、IL-6及DNMT1升高是影响DKD发生的独立危险因素(P<0.05)。24 h UAER、IL-6、DNMT1联合检测诊断DKD的曲线下面积优于单一指标检测。结论 DKD患者DNMT1、IL-6水平升高,24 h UAER、DNMT1、IL-6联合检测可作为临床诊断DKD的良好指标。
Objective To investigate the expression and clinical significance of peripheral blood mononuclear cell DNA methyltransferase 1(DNMT1) and serum interleukin-6(IL-6) in diabetic nephropathy(DKD).Methods A total of150 DKD patients(the DKD group) and 50 healthy subjects(the control group) were selected.Data of IL-6,DNMT1,high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),serum creatinine,fasting blood glucose,estimated glomerular filtration rate(eGFR),24-hour urinary albumin excretion rate(24 h UAER) and other clinical indicators were compared between the two groups.Pearson correlation was used to analyze the correlation between IL-6,DNMT1 and other clinical indicators.Multivariate Logistic regression was used to analyze the influencing factors of DKD.Receiver operating curve(ROC) was used to analyze the diagnostic value of IL-6,DNMT1 and combined detection in DKD.Results Compared with the control group,levels of fasting blood glucose,IL-6,DNMT1,serum creatinine,24 h UAER,LDL-C and glycosylated hemoglobin were higher in the DKD group,but levels of eGFR and HDL-C were lower(P <0.05).IL-6 was positively correlated with DNMT1 in the DKD group(r=0.560,P<0.05),and IL-6,DNMT1 and 24 h UAER were positively correlated(r=0.551,0.570,P <0.05).Elevated 24 h UAER,elevated IL-6 and elevated DNMT1 were independent risk factors for DKD(P <0.05).The combined detection of 24 h UAER,IL-6 and DNMT1 in the area under the surface of DKD was significantly higher than that of single index detection.Conclusion DNMT1 and IL-6 are highly expressed in DKD patients,and the combined detection of 24 h UAER,DNMT1 and IL-6 can be used as a good indicator for clinical diagnosis of DKD.
作者
许莉敏
谢燕
XU Limin;XIE Yan(Department of General Medicine,the First Affiliated Hospital of Soochow University,Suzhou 215031,China)
出处
《天津医药》
CAS
北大核心
2023年第2期194-198,共5页
Tianjin Medical Journal
作者简介
许莉敏(1983),女,主治医师,主要从事肾脏疾病的基础与临床方面研究。E-mail:xulimin5123524@163.com;通信作者:谢燕,E-mail:xieyan005@163.com。
