摘要
本课题组前期研究发现,绿僵菌素A与家蚕Bombyx mori的hemocytin蛋白互作,强烈抑制血淋巴免疫,预示hemocytin可能成为一种杀虫剂的新型作用靶标。因此,进一步了解hemocytin十分必要。Hemocytin是昆虫血淋巴免疫的重要因子,作为一种凝集素,介导血淋巴中的凝血、结节和囊胞化过程,防止表皮破损造成的血淋巴外溢和微生物入侵,并参与对已入侵病原的固定与清除。昆虫的hemocytin一般由3000~4000个氨基酸组成,是一个巨大的多结构域蛋白,含有多个重复排列的结构域,包括FA58C(coagulation factor 5 or 8 C-terminal),VWD(von Willebrand factor type D),TIL(trypsin inhibitor like cysteine rich),VWC(von Willebrand factor type C),CT(C-terminal cystine knot-like),C8(8 conserved cysteine residues),ChtBD2(chitin-binding domain type 2)和MUC(mucin-2 protein WxxW repeating region);不同昆虫间hemocytin的氨基酸序列相似性较小,但其结构域序列具有较高的保守性。Hemocytin由血细胞生物合成,以成熟形式分泌至血淋巴中。Hemocytin是凝血块的主要成分,通过其纤维结构凝聚血细胞和凝血因子形成软凝块封闭伤口,再通过交联作用形成硬凝块和结痂。Hemocytin在结节和囊胞化过程中发挥重要作用,将血细胞、免疫因子和病原体凝聚,最后联合黑化作用隔绝和杀死病原体。总体上,昆虫hemocytin的研究还不够深入。解析hemocytin调控昆虫免疫的分子机理,对于丰富昆虫免疫学基础研究,促进基于hemocytin为靶点的新型杀虫剂研发具有重要意义。
Our research group previously found that destruxin A interacts with the hemocytin of the silkworm,Bombyx mori,and strongly suppresses hemolymph immunity,suggesting that hemocytin may become a novel target for pesticide.Therefore,it is necessary to further understand hemocytin.Hemocytin,as a lectin,is an important factor in insect hemolymph immunity to mediate the process of coagulation,nodulation and encapsulation and prevent hemolymph spillage and microbial invasion caused by epidermal breakage,as well as be involved in the fixation and removal of invaded pathogens.Insect hemocytin generally consists of 3000-4000 amino acids with multiple and repeatedly arranged domains including FA58C(coagulation factor 5 or 8 C-terminal),VWD(von Willebrand factor type D),TIL(trypsin inhibitor like cysteine rich),VWC(von Willebrand factor type C),CT(C-terminal cystine knot-like),C8(8 conserved cysteine residues),ChtBD2(chitin-binding domain type 2),and MUC(mucin-2 protein WxxW repeating region).The amino acid sequence similarity of hemocytin between different insects is quite low,but the domain sequence is highly conserved.Hemocytin is biosynthesized by hemocytes and secreted into the hemolymph in mature form.Hemocytin is the main component of blood clot,forming soft clot by coagulating hemocytes and clotting factors through its fibrous structure to seal the wound,and then forming hard clot and scab through cross-linking.Hemocytin plays an important role in the process of nodulation and encapsulation,agglutinating hemocytes,immune factors and pathogens,and finally combining with melanization to isolate and kill pathogens.Overall,insect hemocytin has not been studied in depth.Analysis of the molecular mechanism of hemocytin regulation of insect immunity is important to enrich the basic research of insect immunology and promote the development of new insecticides based on hemocytin as the target.
作者
王菁菁
胡宏旺
胡琼波
WANG Jing-Jing;HU Hong-Wang;HU Qiong-Bo(College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;College of Horticulture,South China Agricultural University,Guangzhou 510642,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2022年第12期1687-1694,共8页
Acta Entomologica Sinica
基金
国家自然科学基金项目(U1901205)。
作者简介
王菁菁,女,1993年7月生,博士,研究方向为绿僵菌素杀虫机理,E-mail:wangjingjing@scau.edu.cn;通讯作者:胡琼波,E-mail:hqbscau@scau.edu.cn。
