摘要
目的探究补阳还五汤通过PI3K/AKT通路调控自噬抗大鼠脑缺血/再灌注损伤的作用。方法大鼠随机分为5组(n=10):假手术组(Sham)、模型组(Model)、补阳还五汤组(BYHWD)、PI3K抑制剂组(LY294002)与溶媒剂组(Vehicle)。除Sham组外,其余各组均缺血2 h,再灌注72 h造模处理。Zea Longa评分评估神经缺损、TTC检测脑梗死体积、HE观察脑部缺血半暗带(ischemic penumbra,IP)损伤、免疫荧光检测LC3B、Western blot检测PI3K/AKT通路与自噬标志蛋白。结果BYHWD组与Model组相比,大鼠神经功能评分降低、脑梗死体积减小、脑部IP病理损伤减轻,PI3K与p-AKT/AKT表达增加,LC3Ⅱ/Ⅰ降低,p62升高(P<0.05);BYHWD的调控作用被LY294002减弱(P<0.05)。结论补阳还五汤通过激活PI3K/AKT通路抑制细胞自噬,从而减轻大鼠脑缺血/再灌注损伤。
Aim To explore the effect of Buyang Huanwu Decoction on cerebral ischemia-reperfusion injury in rats by regulating autophagy through PI3K/AKT pathway.Methods The rats were randomly divided into five groups(n=10):sham operation group(Sham),model group(Model),Buyang Huanwu Decoction group(BYHWD),PI3K inhibitor group(LY294002)and Vehicle group(Vehicle).Except Sham group,the other groups were treated with 2h ischemia and 72 h reperfusion for modeling.The Zea Longa score was used to assess the neurological defects,HE was used to observe brain injury in the ischemic penumbra(IP),immunofluorescence was employed to detect LC3,and Western blot was used to detect pathway and autophagy marker proteins.Results Compared BYHWD group with model group,the neurological score of rats decreased,cerebral infarction volume decreased,the pathological lesions of brain IP were relieved,PI3K and p-AKT/AKT expression increased,and LC3Ⅱ/Ⅰdecreased and p62 increased(P<0.05).The regulatory effect of BYHWD was weakened by LY294002(P<0.05).Conclusion Buyang Huanwu Decoction alleviates cerebral ischemia-reperfusion injury in rats by activating PI3K/AKT pathway to inhibit autophagy.
作者
单玉栋
赵艳萌
靳晓飞
周晓红
叶佳蓓
马秀娟
田甜
蔡国英
高维娟
SHAN Yu-dong;ZHAO Yan-meng;JIN Xiao-fei;ZHOU Xiao-hong;YE Jia-bei;MA Xiu-Juan;TIAN Tian;CAI Guo-Ying;GAO Wei-juan(Hebei University of Chinese Medicine,Hebei Key Lab of Chinese Medicine Research on Cardio-cerebrovascular Disease,Shijiazhuang 050091,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2023年第2期386-391,共6页
Chinese Pharmacological Bulletin
基金
中央引导地方科技发展资金项目(No 206Z7706G)
河北中医学院科技能力提升项目(No KTY2019049)。
作者简介
单玉栋(1994-),男,硕士生,研究方向:缺血性脑血管病的中医药防治,E-mail:shanyudong68@163.com;通信作者:高维娟(1966-),女,博士,教授,博士生导师,研究方向:脑血管病的发生机制及中医药防治,E-mail:gwj6088@163.com。
