摘要
目的 研究脑栓通胶囊对局灶性脑缺血再灌注模型大鼠血管新生的影响。方法 用线栓法制备局灶性脑缺血再灌注大鼠模型,术后随机分为假手术组、模型组和脑栓通胶囊小(1.05 g/kg)、大(2.62 g/kg)剂量给药组,术后24 h开始给予脑栓通胶囊,每天灌胃1次,连续给药7 d。采用mNss法评估各组大鼠的神经功能缺损情况,TTC染色检测脑梗死体积百分比,Western blot检测大鼠大脑皮层血管新生相关蛋白的表达,免疫荧光染色检测新生血管。结果 术后7 d,与假手术组相比,模型组mNss评分和脑梗死体积显著增加(均P<0.001);与模型组相比,小剂量给药组和大剂量给药组神经功能损伤有显著改善(P<0.05),大剂量给药组脑梗死体积显著减少(P<0.05)。与模型组相比,小剂量给药组和大剂量给药组血管内皮生长因子(vascular endothelial growth factor, VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor, bFGF)表达水平均显著增加(VEGF:P<0.05,P<0.001;bFGF:P<0.05,P<0.001);与模型组相比,小剂量给药组和大剂量给药组血管新生数量显著增加(P<0.05,P<0.001)。结论 脑栓通胶囊可以通过促进血管新生改善大鼠缺血后神经功能。
Objective To study the effect of Naoshuantong capsule on angiogenesis in rats with focal cerebral ischemia reperfusion.Method Healthy adult male SD rats were induced to develop occlusion of the middle cerebral artery by suture embolus, Then, the rats were randomly divided into the sham group, a model group, and a low-dose Naoshuantong capsule and high-dose Naoshuantong capsule groups(1.05, 2.62 g/kg, respectively). Naoshuantong capsule was administered once per day for 7 days, from 24 h after surgery a day.To examine the recovery of neurological function, we measured modified neurological severity score(mNss) method;the infarction volume was measured by 2, 3, 5-triphenyltetrazolium chloride(TTC) staining technique;Western blot was used to detect the expression of angiogenesis related proteins in rat cerebral cortex;Angiogenesis was detected by immunofluorescence staining.Result Compared with those in the sham group, the scores of neurological behavior and the cerebral infarction volume ratios in the model group were significantly increased(P<0.001). Compared with that in the model group, the low and high-dose Naoshuantong capsule groups exhibited a dramatic improvement of the neurological behavior scores(P<0.05), Naoshuantong capsule high-dose groups exhibited a significant reduction in the volume of cerebral infarction(P<0.05). Compared with the model group, the expression of cortical angiogenesis-related proteins vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) in the Naoshuantong capsule low and high-dose groups were significantly increased(VEGF:P<0.05,P<0.001;bFGF:P<0.05,P<0.001);Compared with the model group, the number of Lectin+/Ki67+cellls were significantly increased(P<0.05,P<0.001).Conclusion Naoshuantong capsule may promote angiogenesis to improve neurological function after ischemia in rats.
作者
郭敏
刘婷婷
吴铮
田欣
祝自新
郑文荣
王宇峰
郑瑞芳
邢建国
孙芳玲
王文
GUO Min;LIU Tingting;WU Zheng;TIAN Xin;ZHU Zixin;ZHENG Wenrong;WANG Yufeng;ZHENG Ruifang;XING Jianguo;SUN Fangling;WANG Wen(Experimental Animal Center,Xuanwu Hospital of Capital Medical University,Beijing 100053,China;Beijing Municipal Geriatric Medical Research Center,Beijing 100053,China;Stroke Institute,Beijing Institute for Brain Disorders,Beijing 100053,China;Xinjiang Uygur Autonomous Region Institute of Materia Medica,Urumqi 830002,China)
出处
《实验动物科学》
2022年第5期20-25,共6页
Laboratory Animal Science
基金
国家自然科学基金(82173795)。
关键词
脑栓通胶囊
脑缺血再灌注
神经功能
脑梗死体积
血管新生
Naoshuantong capsule
cerebral ischemia-reperfusion
neurological function
cerebral infarction volume
angiogenesis
作者简介
郭敏(1993-),女,湖北襄阳人,硕士研究生,研究方向:药理学.E-mail:qgxar@qq.com;通信作者:孙芳玲(1985-),女,博士,副研究员,研究方向:脑重大疾病再生修复治疗,E-mail:sun_fangling@163.com;王文(1968-),男,博士,研究员,研究方向:心脑血管病再生修复治疗.E-mail:lzwwang@163.com。
