摘要
利用碳量子点(CQDs,受体)与ZnSe量子点(供体)建立环境友好型ZnSe-CQDs荧光共振能量转移体系来测定盐酸强力霉素(DOX)。结果表明:在激发波长340 nm下,于pH=7.2的Tris-HCl的缓冲溶液中,CQDs与ZnSe量子点可在5 min内发生有效的能量转移,导致波长380 nm处ZnSe量子点的荧光猝灭,波长450 nm处CQDs的荧光增强。DOX的加入可以有效猝灭ZnSe-CQDs体系的荧光,且DOX浓度在0.025μg/mL~10.00μg/mL间与体系在波长380 nm和450 nm荧光强度的比值(I 380/I 450)呈良好的线性关系(r=0.9987)。基于此建立了测定DOX的新方法,方法的检出限(3σ/k,n=11)达7.51 ng/mL。将所建立的方法应用于人尿液样品中盐酸强力霉素的检测,回收率为98.00%~106.3%,相对标准偏差(RSD,n=6)小于6.1%。
In this paper,an environmental-friendly fluorescence resonance energy transfer system based on carbon quantum dots(CQDs,donor)and ZnSe quantum dots(acceptor)was constructed for the detection of doxycycline hyclate(DOX).Results showed that under the excitation of 340 nm,efficiency fluorescence resonance energy transfer occurred between CQDs and ZnSe within 5 min in pH 7.2 Tris-HCl buffer solution,which resulted in fluorescence quenching of ZnSe at 380 nm and fluorescence enhancement of CQDs at 450 nm.With the addition of DOX,an efficiency fluorescence quenching of the ZnSe-CQDs system was observed,and the fluorescence ratio of I 380/I 450 was in a linear relationship with DOX concentration in the range of 0.025μg/mL-10.00μg/mL(R=0.9987).Based on this,a new method for DOX detection was constructed,and the limited of detection was calculated to be 7.51 ng/mL(n=11).Common antibiotics,ions and the main components in urea showed little influence on DOX detection,indicating a good selectivity for the proposed probe.The newly established method was employed to detect DOX in urea samples with recovery ranging from 98.00%-106.3%and RSD less than 6.1%(n=6).
作者
刘长青
廖楚健
周素莲
易忠胜
陶慧林
LIU Changqing;LIAO Chujian;ZHOU Sulian;YI Zhongsheng;TAO Huilin(Qinghai Nonferrous First Geological Exploration Institute,Xining,810006;Gongcheng Maternal and Child Health Hospital,Guilin 542500;Guilin University of Technology at Nanning,Nanning,530001;College of Chemistryand Bioengineering,Guilin University of Technology,Guilin 541004)
出处
《分析科学学报》
CAS
CSCD
北大核心
2022年第6期705-710,共6页
Journal of Analytical Science
基金
国家自然科学基金(No.22166015,21866011)。
关键词
碳量子点
ZNSE量子点
荧光共振能量转移
盐酸强力霉素
Carbon quantum dots
ZnSe quantum dots
Fluorescence resonance energy transfer
Doxycycline hyclate
作者简介
通讯作者:周素莲,女,硕士,研究员,研究方向:光谱分析.E-mail:191747306@qq.com。
