摘要
为判别北方稻蟹共作模式下中华绒螯蟹的地理来源,采用电感耦合等离子体-质谱仪(ICP-MS)对黑龙江省、吉林省和辽宁省共36个中华绒螯蟹样本中的V、Cr、Mn等16种元素组分进行定量分析及数据差异性分析。结果表明,3个省份的中华绒螯蟹样本中V、Cr、Ni、Mn、Zn、Ba、Fe、Mo、Cd、As、Pb和U元素的含量差异显著(P<0.05),大部分元素之间表现出极显著的相关关系。基于矿物元素含量特征,结合主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)和逐步线性判别分析(S-LDA)等方法,分别对不同省份的中华绒螯蟹样本进行产地判别。结果显示:PCA方法仅能区分部分样本,而OPLS-DA和S-LDA方法初始判别正确率均达100%,交叉验证OPLS-DA预测能力为71.4%,S-LDA判别准确率高达97.2%,为最优判别方法。Zn、Cr、Pb元素含量是3省份中华绒螯蟹样本溯源的重要变量。结论:基于元素指纹图谱结合化学计量学分析,能够对东北稻蟹共作模式下中华绒螯蟹原产地进行有效判别。
In order to identify the geographical origin of Eriocheir sinensis under the co-cropping mode of Northern Rice crab,the 16 elements of V,Cr,Mn and other 16 elements in 36 samples of Eriocheir sinensis in Heilongjiang,Jilin and Liaoning province were quantitatively detected by ICP-MS,and the data were analyzed.The results showed that the contents of V,Cr,Ni,Mn,Zn,Ba,Fe,Mo,Cd,As,Pb and U in the samples of Eriocheir sinensis were significantly different(P<0.05).Most of the elements showed a very significant correlation.Based on the mineral element content characteristics,the origin of Eriocheir sinensis samples in different provinces was determined by PCA,OPLS-DA and S-LDA.The results showed that PCA could only distinguish some samples,while the initial discrimination accuracy of OPLS-DA and S-LDA methods is 100%,the prediction ability of OPLS-DA was 71.4%,and the accuracy of S-LDA was 97.2%,which was the best method.The contents of Zn,Cr and Pb were important variables for tracing the samples of Eriocheir sinensis in three provinces.The results showed that the effective identification of the origin of Eriocheir sinensis under the co-cropping mode of rice crab in Northeast China can be realized based on the combination of element fingerprint and chemometrics analysis.
作者
白淑艳
王鹏
陈中祥
吴松
郝其睿
高磊
杜宁宁
覃东立
Bai Shuyan;Wang Peng;Chen Zhongxiang;Wu Song;Hao Qirui;Gao Lei;Du Ningning;Qin Dongli(Heilongjiang River Fishery Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070;Supervision,Inspection and Testing Center for Fishery Environment and Aquatic Products(Harbin),Ministry of Agriculture and Rural Affairs,Harbin 150070)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2022年第12期303-312,共10页
Journal of Chinese Institute Of Food Science and Technology
基金
中央公益性科研院所基本科研业务费项目(HSY202110Q)
国家重点研发计划项目(2020YFD0900301)
国家公益性行业(农业)科研专项(201503108)。
关键词
稻田养殖
中华绒螯蟹
产地判别
矿物元素
rice crab farming system
Eriocheir sinensis
origin identification
mineral elements
作者简介
第一作者:白淑艳,女,硕士,助理研究员;通信作者:覃东立,E-mail:qdl978@163.com。
