摘要
为探究黄芩叶斑病的病原种类,筛选有效的杀菌剂,本研究从甘肃省定西市陇西县黄芩主产区采集黄芩叶斑病病叶,采用组织分离法进行病原物的分离,通过病原菌的形态特征并结合rDNA转录间隔区(rDNA-ITS)、过敏原基因(Alt a1)、质膜腺苷三磷酸基因(ATPase)和组蛋白基因(His 3)4个基因序列对病原菌进行种类鉴定,采用菌丝生长速率法测定4种杀菌剂对病原菌的抑菌作用。结果表明,引起陇西县黄芩叶斑病的病原菌为茄链格孢(Alternaria solani)和细极链格孢(Alternaria tenuissima),其中细极链格孢为优势病原菌;室内毒力测定表明,97%咯菌腈的抑菌作用最强,对茄链格孢(A.solani)和细极链格孢(A.tenuissima)均有较好的抑菌作用,EC_(50)分别为0.06和0.04 mg·L^(-1)。本研究在国内首次报道了茄链格孢(A.solani)和细极链格孢(A.tenuissima)是引起黄芩叶斑病的病原菌,可为科学诊断该病害、研究其发生规律和田间防治提供理论基础和科学依据。
In order to determine the pathogen species and screen the effective fungicides for Scutellaria baicalensis leaf spot disease,the leaves infected by spot disease were collected from Longxi county,Dingxi,Gansu province and were used for the isolation of pathogens.The isolates were identified based on their morphological characteristics and the sequences of internal transcription spacer,allergen gene,plasma membrane adenosine triphosphate ATPase and histone 3.The results showed that the disease pathogens were identified as Alternaria solani and Alternaria tenuissima,in which A.tenuissima was the dominant species.Meanwhile,the mycelia growth rate was applied to test the toxicity of four fungicides and 97%Fludioxonil exhibited the strongest inhibitory activity on A.solani and A.tenuissima with EC_(50) at 0.06 and 0.04 mg·L^(-1),respectively.In summary,this is the first report on leaf spot in S.baicalensis caused by A.solani and A.tenuissima in China,which provides a theoretical basis for the diagnosis of disease,understanding of the disease development,prevention and control of the disease in field.
作者
蒋晶晶
赵娇娇
陈爱昌
曹素芳
李继平
李青青
徐美蓉
漆永红
JIANG Jingjing;ZHAO Jiaojiao;CHEN Aichang;CAO Sufang;LI Jiping;LI Qingqing;XU Meirang;QI Yonghong(Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070;College of Horticulture,Gansu Agricultural University,Lanzhou,Gansu 730070;Plant Protection Station,Dingxi,Gansu 743000;Institute of Fruit and Floriculture Research,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070;Institute of Agricultural Quality Standards and Testing Technology,Gansu Acedemy of Agricultural Sciences,Lanzhou,Gansu 730070)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第11期2166-2174,共9页
Journal of Nuclear Agricultural Sciences
基金
甘肃省农业科学院科研条件建设及成果转化项目(2020GAAS25,2020GAAS38)
国家重点研发计划(2018YFD0201100)
甘肃省科技计划项目(20YF3NA021)。
关键词
黄芩
叶斑病
细极链格孢
茄链格孢
毒力测定
Scutellaria baicalensis Georgi
leaf spot disease
Alternaria tenuissima
Alternaria solani
toxicity testing
作者简介
蒋晶晶,女,助理研究员,主要从事经济作物病害研究。E-mail:jingjingziyu@163.com;通讯作者:徐美蓉,女,实验师,主要从事农产品质量安全与农药残留分析研究。E-mail:xumeirong@gsagr.ac.cn;通讯作者:漆永红,男,副研究员,主要从事经济作物病害研究。E-mail:qiyonghong920@gsagr.ac.cn。
