摘要
[目的]优化木犀草素脂质体的冻干工艺,考察其对LX-2细胞增殖的影响。[方法]采用冷冻干燥法制备木犀草素脂质体冻干粉,以外观、再分散性、粒径及包封率为指标,通过单因素考察和Box-Behnken响应面法优选最佳冻干工艺,MTT法检测其对LX-2细胞增殖的影响。[结果]最佳冻干工艺为预冻温度-80℃,预冻时间12 h,干燥时间24 h,冻干保护剂为总量8%的蔗糖-乳糖-甘露醇(1.0∶1.0∶2.0)联合使用;与冻干前比较,冻干粉在4℃下30 d内较稳定;脂质体对LX-2细胞的抑制作用强于原药(P<0.01),并呈浓度和时间依赖性。[结论]按最优工艺制备的脂质体冻干粉包封率为89.03%,与预测值90.12%吻合度高达98.79%;且脂质体对LX-2细胞增殖的抑制率显著高于原药(P<0.01)。
[Objective]To optimize the freeze-dried process of luteolin liposome and investigate its effect on the proliferation of LX-2 cells.[Method]Luteolin liposomes freeze-dried powder were prepared by the method of freeze-drying.The best prescription process was selected by the single factor experiment and Box-Behnken response surface methodology with indexes of the appearance,redispersibility,particle size and encapsulation efficiency and MTT assay to detect its effect on the proliferation of LX-2 cells.[Result]The best prescription was prefreeze-temperature-50℃,prefreeze-time 12 hours,dry-time 24 hours,The freeze-dried protective agent was combined use of sucrose-lactose-mannitol(1.0∶1.0∶2.0),and the total content was 8%.Compared with before freeze-drying,liposomes freeze-dried power had good stability within 30 days at 4℃.The inhibitory effect of liposomes on LX-2 cells is stronger than that of raw medicine(P<0.01)in concentration dependent and time-dependent manners.[Conclusion]The encapsulation efficiency of the freeze-dried powder of liposome prepared by the optimal process was 89.03%,which was 98.79%in agreement with the predicted value of 90.12%;and the inhibition rate of liposomes on LX-2 cell proliferation was significantly higher than that of the original drug(P<0.01).
作者
陆倩
国小利
杨雅欣
何迅
张金娟
廖尚高
LU Qian;GUO Xiao-li;YANG Ya-xin;HE Xun;ZHANG Jin-juan;LIAO Shang-gao(School of Pharmacy,Guizhou Medical University;Functional Laboratory,School of Basic Medicine,Guizhou Medical University,Guiyang 550025,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM,Ministry of Education/Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,China)
出处
《生物技术》
CAS
2022年第5期598-604,629,共8页
Biotechnology
基金
贵州省高层次创新型人才百层次人才项目(黔科合平台人才[2020]6011)
贵州省中药、民族药活性物质筛选新技术开发应用创新人才团队资助项目(黔科合平台人才[2020]5006)。
作者简介
陆倩(1997-),女,贵州省盘县人,硕士,研究方向:天然药物活性成分分析,E-mail:1396892346@qq.com。;通讯作者:廖尚高(1972-),男,博士,教授,博导,研究方向:中草药药效物质及其开发应用,发表论文100余篇,其中SCI论文50余篇,获专利授权7项,E-mail:lshangg@163.com。
