摘要
[目的]旨在筛选最适合钩藤幼苗生长发育及生物碱积累的培养基。[方法]以6种培养基(WPM、1/2MS、MS、White、B5、N6)培育的钩藤幼苗为研究对象,统计各个培养基中钩藤种子的萌发率并记录钩藤幼苗的表型;利用丙酮乙酰混合液法测定钩藤幼苗叶片的叶绿素a、叶绿素b和叶绿素总含量;运用HPLC分别测定钩藤幼苗叶片的钩藤碱和异钩藤碱含量。[结果]WPM培养基培育的钩藤种子萌发率最高,达42%,其余5种培养基的萌发率均低于30%,WPM培养基培育的钩藤幼苗的表型、鲜重和干重均为最优;1/2MS培养基培育的钩藤幼苗积累的光合色素含量最多,除叶绿素b含量与WPM有显著差异外,叶绿素a和叶绿素总量与WPM培养基均无显著差异;WPM培养基培育的钩藤幼苗叶片的钩藤碱和异钩藤碱含量均最高,是White培养基1.57和1.31倍。[结论]WPM培养基为钩藤种子萌发、生长发育以及生物碱积累的最适培养基,为钩藤优质微繁殖种苗的培育和药用成分的代谢合成的研究奠定了基础。
[Objective]To screen the optimum medium for growth and alkaloid accumulation of U.rhynchophylla seedlings.[Method]In this study,U.rhynchophylla seedlings cultured in 6 media(WPM,1/2MS,MS,White,B5,N6)were used as the research subjects.The germination rate of U.rhynchophylla seeds in each medium were calculated and the phenotypes of U.rhynchophylla seedlings were recorded.The contents of chlorophyll a,chlorophyll b and total chlorophyll in U.rhynchophylla seedling leaves were determined by acetone acetyl mixture method.The contents of rhynchophylline and isorhynchophylline in leaves of U.rhynchophylla seedlings were determined by HPLC.[Results]The germination rate of U.rhynchophylla seedlings cultured on WPM medium was the highest(42%),and the germination rate of the other five media was lower than 30%.The phenotype,fresh weight and dry weight of U.rhynchophylla seedlings cultured on WPM medium were the best.The photosynthetic pigment content of U.rhynchophylla seedlings cultured on 1/2MS medium was the highest,and the chlorophyll a and total chlorophyll content had no significant difference with WPM medium except the chlorophyll b content.The contents of rhynchophylline and isorhynchophylline in leaves of U.rhynchophylla seedlings cultured in WPM medium were the highest,which were 1.57 and 1.31 times of those in White medium.[Conclusion]WPM medium was the most suitable medium for seeds germination,growth and development as well as alkaloid accumulation of U.rhynchophylla,which laid a foundation for the cultivation of high-quality micropropagation seedlings and the study on the metabolism and synthesis of medicinal components.
作者
路星星
谭艾娟
强玮
周浩
敖雯雯
吕世明
张明生
LU Xing-xing;TAN Ai-juan;QIANG Wei;ZHOU Hao;AO Wen-wen;LYU Shi-ming;ZHANG Ming-sheng(Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China;College of Animal Sciences,Guizhou University,Guiyang 550025,China)
出处
《生物技术》
CAS
2022年第5期569-575,共7页
Biotechnology
基金
贵州省省级科技计划项目(黔今科合基础-ZK[2022]一般096)
贵州大学培育项目(贵大培育[2019]51号)
贵州大学引进人才科研项目(贵大人基合字[2017]58)
贵州省中药材现代产业技术体系建设项目(GZCYTX-02)
国家喀斯特石漠化防治工程技术研究中心建设(2012FU125X13)
贵州省生物学一流学科建设项目(GNYL[2017]009)。
关键词
钩藤
培养基
光合色素
钩藤碱
异钩藤碱
U.rhynchophylla
medium
photosynthetic pigment
rhynchophylline
isorhynchophylline
作者简介
路星星(1996-),女,贵州省毕节人,硕士,研究方向:分子生物学,发表论文2篇,E-mail:825280760@qq.com。;通讯作者:谭艾娟(1963-),女,贵州省贵阳人,硕士,教授,研究方向:生物化学与分子生物学,近5年发表论文20余篇,E-mail:772771868@qq.com。;通讯作者:强玮(1988-),男,贵州省贵阳人,博士,副教授,研究方向:药用植物代谢工程与合成生物学,近5年发表论文10余篇,E-mail:qiangwei880311@126.com。
