摘要
Diabetes treatment and rehabilitation are usually a lifetime process.Optogenetic engineered designer cell-therapy holds great promise in regulating blood glucose homeostasis.However,portable,sustainable,and long-term energy supplementation has previously presented a challenge for the use of optogenetic stimulation in vivo.Herein,we_purpose a self-powered optogenetic system(SOS)for implantable blood glucose control.The SOS consists of a biocompatible far-red light(FRL)source,FRL-triggered transgene-expressing cells,a power management unit,and a flexible implantable piezoelectric nanogenerator(i-PENG)to supply long-term energy by converting biomechanical energy into electricity.Our results show that this system can harvest energy from body movement and power the FRL source,which then significantly enhanced production of a short variant of human glucagon-like peptide 1(shGLP-1)in vitro and in vivo.Indeed,diabetic mice equipped with the SOS showed rapid restoration of blood glucose homeostasis,improved glucose,and insulin tolerance.Our results suggest that the SOs is sufficiently effective in self-powering the modulation of therapeutic outputs to control glucose homeostasis and,furthermore,present a new strategy for providing energy in optogenetic-based cell therapy.
出处
《Research》
EI
CAS
CSCD
2022年第4期295-307,共13页
研究（英文）
基金
We are grateful to all the laboratory members for their cooperation in this study.This work was financially supported by grants from the National Key R&D Program of China,Synthetic Biology Research(no.2019YFA0904500)
the National Natural Science Foundation of China(nos.82102231,31971346,61875015,31861143016,U20A20390,11827803,and T2125003)
the Science and Technology Commission of Shanghai Municipality(no.22N31900300)
Beijing Natural Science Foundation(JQ20038,L212010)
China Postdoctoral Science Foundation(2020M680302,2021T140041)
the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16021101)
We also thank the ECNU Multifunctional Platform for Innovation(011)for supporting the murine experiments and the Instruments Sharing Platform of the School of Life Sciences,ECNU.
作者简介
Correspondence should be addressed to:Yubo Fan,yubofan@buaa.edu.cn;Correspondence should be addressed to:Zhou Li,zli@binn.cas.cn;Correspondence should be addressed to:Haifeng Ye,hfye@bio.ecnu.edu.cn。
