摘要
骨感染因治疗困难、预后差等特点,是临床严重的感染性疾病之一,故早期准确诊断骨感染是治疗成功的关键。传统的检测方法速度慢、阳性率低,因此急需找到更好的检测技术来确定骨感染的病原微生物。近年来,宏基因组学第二代测序技术因其准确、快速、高效,具有全面检测病原微生物的优势,已经用于临床感染性疾病的诊断,其也可以作为一种有效诊断骨感染的工具。本文主要就宏基因组学第二代测序技术在骨感染诊断应用中的优势、不足及发展方向进行综述。
Bone infection is a serious infectious disease in clinical practice due to its difficult treatment and poor prognosis.Therefore,early accurate diagnosis of bone infection is the key for successful treatment.The traditional detection methods is time consuming with lower positive rate.Therefore,it is urgent to find better detection techniques to identify the pathogenic microorganisms of bone infection.In recent years,metagenomic second-generation sequencing technology has been widely used in the diagnosis of clinical infectious diseases because of its advantages of accurate,rapid,efficient and comprehensive diagnosis of pathogenic microorganisms,and can be used as an effective tool for the diagnosis of bone infections.This paper mainly reviews the advantages,disadvantages and development direction of metagenomics next-generation sequencing technology in the diagnosis of bone infection.
作者
马宇杰
侯卫坤
许珂
杨明义
万贤杰
许鹏
Ma Yujie;Hou Weikun;Xu Ke;Yang Mingyi;Wan Xianjie;Xu Peng(Department of Osteonecrosis and Joint Recon-Struction,Honghui Hospital,Xi′an Jiaotong University,Xi′an 710054,Chnia;Medical School of Yan′an University,Yan′an 716000,China)
出处
《国际外科学杂志》
2022年第11期780-784,共5页
International Journal of Surgery
基金
国家自然科学基金(82072432)
中国博士后科学基金(2020M673454)。
关键词
感染
骨疾病
感染性
诊断
宏基因组学第二代测序
Infection
Bone diseases,infectious
Diagnosis
Metagenomics next-generation sequencing
作者简介
通信作者:许鹏,Email:sousou369@163.com。
