摘要
探索荒漠固沙植物根际土壤nif H基因丰度信息和固氮菌群落结构组成和多样性,可为丰富荒漠区微生物资源库提供基础资料。通过实时荧光定量PCR和高通量测序方法,分析了宁夏荒漠区5个柠条林地根际土壤nif H基因丰度和固氮菌群落结构组成,并结合分离培养方法对柠条根际固氮菌进行筛选和多样性研究。结果表明,5个柠条群落根际土壤nif H基因的拷贝数存在较大的差异,罗山林地(LS)nif H基因拷贝数最高,沙坡头林地(SPT)最低。柠条根际土壤共检测到固氮菌7门12纲26科30属。在科属水平上,沙坡头林地固氮菌群落组成差异较大,未分类的变形杆菌和叶杆菌科分别占25.54%和22.77%,红螺菌科占17.43%,中慢生根瘤菌属是其优势属,相对丰度为22.77%;其余4个林地均以红螺菌科、斯科曼氏球菌属为优势类群,相对丰度分别为23.71%-73.45%和23.19%-71.14%,在罗山林地中占比最高。分离出的固氮菌隶属于15个属,除常见的假单胞菌属、根瘤菌属和肠杆菌属等固氮类群外,还包括勒克氏菌属、金黄杆菌属和寡养单胞菌属等,多样性十分丰富。
Exploring the abundance information of nif H gene and the composition and diversity of nitrogen-fixing bacterial community in the rhizosphere soil of the desert sand-fixing plant may provide basic information for enriching the microbial resource pool in desert areas. Realtime quantitative PCR and high-throughput sequencing were applied to analyze the abundance of nif H gene and the community composition of nitrogen-fixing bacteria in the rhizosphere soil of 5 shrublands of Caragana spp. growing in the desert grassland of Ningxia. The method of isolation and culture were to screen nitrogen-fixing bacteria in the rhizosphere soil of Caragana spp. and to study their diversities. The results showed that the copy number of nif H gene in the 5 rhizosphere soil samples were significantly different,the highest in Luoshan(LS)and the lowest in Shapotou(SPT). A total of 7 phyla,12 classes,26 families and 30 genera of nitrogen-fixing bacteria were detected in the rhizosphere soil of Caragana spp. At the family and genus level,the community composition of nitrogen-fixing bacteria in Shapotou sample was quite different. Unclassified Proteobacteria and Phyllobacteriaceae accounted for 25.54% and 22.77%,respectively,Rhodospirillaceae accounted for 17.43%,and Mesorhizobium were dominant genus,with a relative abundance of 22.77%;the other four soil samples were dominated by Rhodospirillaceae and Skermanella,with relative abundances of 23.71%-73.45% and 23.19%-71.14%,respectively,and it accounted for the highest proportion in Luoshan sample. The isolated nitrogen-fixing bacteria belong to 15 genera,in addition to the common nitrogen-fixing groups such as Pseudomonas,Rhizobium and Enterobacter,also including Leclercia,Chryseobacterium,Stenotrophomonas,etc.,the diversity is abundant.
作者
刘爽
姚佳妮
沈聪
代金霞
LIU Shuang;YAO Jia-ni;SHEN Cong;DAI Jin-xia(School of Life Sciences,Ningxia University,Yinchuan 750021)
出处
《生物技术通报》
CAS
CSCD
北大核心
2022年第12期252-262,共11页
Biotechnology Bulletin
基金
国家自然科学基金项目(32160023,31760027)
宁夏自然科学基金项目(2020AAC03104)。
关键词
柠条
nif
H基因
固氮菌群落
多样性
Caragana spp.
nifH gene
nitrogen-fixing bacteria community
diversity
作者简介
刘爽,女,硕士研究生,研究方向:微生物资源开发与利用,E-mail:1849468755@qq.com;通讯作者:代金霞,女,博士,教授,研究方向:微生物资源开发与利用,E-mail:daijx@163.com。
