泽泻叶三萜的制备及其对磺胺间甲氧嘧啶钠在大鼠体内代谢的影响

Preparation of Triterpenoids from Leaves of Alismatis Rhizoma and Its Effects on the Metabolism of Sulfamonomethoxine Sodium in Rats

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摘要【目的】优化泽泻叶总三萜提取的最佳酶解工艺,并探究得到的总三萜提取物对磺胺间甲氧嘧啶钠在大鼠体内代谢的影响。【方法】以总三萜提取率为指标,在单因素的基础上,采用响应面法优选出酶解的最佳工艺参数。通过测定血药浓度,考察总三萜提取物对磺胺间甲氧嘧啶钠在大鼠体内代谢的影响。【结果】泽泻叶总三萜提取的最优酶解工艺为纤维素酶和木瓜蛋白酶比例1∶2,酶用量1.20%,料液比1∶25,酶解温度50℃,酶解时间70 min。泽泻叶总三萜提取物能够加速磺胺间甲氧嘧啶钠在大鼠体内的代谢,降低药物残留。【结论】该酶解工艺稳定可行,具有推广应用价值。所得的泽泻叶总三萜提取物具有加速磺胺间甲氧嘧啶钠代谢的作用,可缩短兽医临床磺胺间甲氧嘧啶钠的休药期,减少抗生素残留。【Objective】To optimize the optimal enzymolysis process for the extraction of total triterpenes from leaves of Alismatis Rhizoma and to explore the effect of total triterpene extract on the metabolism of sulfamonomethoxine sodium in rats.【Method】Taking the extraction rate of total triterpenes as the indicator,the optimal process parameters were selected by response surface methodology on the basis of single factor. The effect of total triterpenes on the metabolism of sulfamonomethoxine sodium in rats was detected by determining the plasma concentration.【Result】The optimal enzymolysis process for the extraction of total triterpenes from leaves of Alismatis Rhizoma was finally revised as follows:the ratio of cellulase to papain 1∶2,the amount of enzyme 1.20%,the ratio of material to liquid 1∶25,the enzymolysis temperature 50℃,the enzymolysis time 70 min. The total triterpenoids from leaves of Alismatis Rhizoma can accelerate the metabolism of sulfamonomethoxine sodium in rats and reduce its residues.【Conclusion】The enzymolysis process is stable and feasible,and has the value of popularization and application. The total triterpenoids extract from leaves of Alismatis Rhizoma can accelerate the metabolism of sulfamonomethoxine sodium,shorten the withdrawal period of sulfamonomethoxine sodium in veterinary clinic and reduce the residue of antibiotics.

作者王榆鑫陶梦婷张淇赵宇哲兰坤陶超李丽霞 WANG Yuxin;TAO Mengting;ZHANG Qi;ZHAO Yuzhe;LAN Kun;TAO Chao;LI Lixia(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China)

机构地区四川农业大学动物医学院

出处《四川农业大学学报》 CSCD 北大核心 2022年第6期920-927,共8页 Journal of Sichuan Agricultural University

基金国家现代农业产业技术体系四川兽药创新团队计划(CARS-SVDIP) 四川省中医药管理局面上项目(2021MS219) 成都市科技局技术创新研发项目(2021-YF05-00078-SN)。

关键词泽泻叶总三萜磺胺间甲氧嘧啶钠复合酶酶解工艺响应面法固相萃取-高效液相色谱法 total triterpenoids from leaves of Alismatis Rhizoma sulfamonomethoxine sodium complex enzyme enzymolysis process response surface methodology SPE-HPLC

分类号 S859.7 [农业科学—临床兽医学]

作者简介王榆鑫,本科生;责任作者:李丽霞,博士,讲师,主要从事中药品种、品质和资源开发研究,E-mail:lilixia905@163.com。