摘要
为了研究月见草Oenothera biennis L.的化学成分,采用硅胶、Sephadex LH-20凝胶、MCI gel CHP-20等柱色谱并结合半制备高效液相等分离技术,利用现代波谱学手段进行化合物结构鉴定。从月见草50%丙酮提取物中分离得到10个化合物,分别鉴定为dimethyl 3-lactyl shikimate(1)、methyl shikimate(2)、3,3′,4-O-三甲基鞣花酸(3)、3,3′-二甲氧基-鞣花酸(4)、3,4,3′-三甲氧基鞣花酸(5)、alternariol 9-methyl ether(6)、2,3-二羟基-1-(4-羟基-3,5-二甲氧基苯基)-1-丙酮(7)、scirpyrone H(8)、dimethoxydimethylphthalide(9)、clearanol C(10)。其中化合物1为新化合物,化合物2~10均为首次从该植物中分离得到。化合物2和3可显著提高TGF-β1诱导BEAS-2B细胞的活力,推测其具有潜在的肺保护作用。
To study the chemical constituents of Oenothera biennis L..The chemical constituents from 50%acetone extract of the O.biennis were isolated by repeated chromatograph with silica gel,Sephadex LH-20,MCI gel CHP-20 and semi-preparative RP-HPLC.The structure of the separated chemical components was identified by modern spectroscopy identification.Ten compounds were isolated from the ethyl acetate fraction of 50%acetone extract of O.biennis,and identified as dimethyl 3-lactyl shikimate(1),methyl shikimate(2),3,3′,4-O-triomethylellagic acid(3),3,3′-dimethylellagic acid(4),3,4,3′-trimethoxy flavellagic acid(5),alternariol 9-methyl ether(6),2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(7),scirpyrone H(8),dimethoxydimethylphthalide(9),clearanol C(10).Compound 1 was a new shikimate derivative and compounds 2-10 were newly isolated from the O.biennis.Compounds 2 and 3 could significantly increase the viability of BEAS-2B cells induced by TGF-β1,showing potent lung protection.
作者
刘娟娟
张靖柯
曾梦楠
李孟
朱登辉
魏俊俊
郑晓珂
冯卫生
LIU Juan-juan;ZHANG Jing-ke;ZENG Meng-nan;LI Meng;ZHU Deng-hui;WEI Jun-jun;ZHENG Xiao-ke;FENG Wei-sheng(College of Pharmacy,Henan University of Chinese Medicine;The Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第12期2034-2039,共6页
Natural Product Research and Development
基金
河南省重大科技专项(171100310500)
国家重点研发计划-中医药现代化研究(2019YFC1708802)。
作者简介
通信作者:冯卫生,Tel:86-371-60190296,E-mail:fwsh@hactcm.edu.cn。
