摘要
目的研究四连接同源(FJX1)基因在结肠癌中的表达情况,分析其表达与患者临床特征及预后的关系,探讨FJX1的分子作用机制。方法在TCGA官方数据库中下载结肠癌转录组测序数据和患者的临床资料。使用R4.1.2软件提取FJX1基因在结肠癌组织与正常组织中的表达量,使用Mann-Whitney U检验分析FJX1基因在两组之间的表达差异,分析FJX1基因表达与患者临床病理特征的关系。使用Kaplan-Meier法分析FJX1基因表达与患者总体生存率的相关性。采用Cox回归分析FJX1基因在结肠癌中的分子预后价值。采用基因富集分析探索FJX1基因在结肠癌中的作用机制。结果与正常组织相比,FJX1基因表达在结肠癌组织中明显上调(P<0.001)。FJX1基因表达水平在患者不同Stage分期、M分期中比较,差异均有统计学意义(均P=0.038)。高表达FJX1基因患者的总体生存率低于低表达的患者(P<0.05)。多因素Cox回归结果显示,FJX1基因可以作为结肠癌患者预后的独立影响因素(HR=1.133,95%CI:1.025~1.253,P=0.015)。基因富集结果显示,FJX1基因在体内可以通过细胞黏附、细胞因子作用、ECM受体作用以及癌症相关信号途径发挥其生物学功能。结论FJX1基因在结肠癌组织中表达上调,与患者的恶性进展特征相关,可作为结肠癌潜在的分子标志物。
Objective To explore the expression level of four-jointed box kinase 1(FJX1)gene in colon cancer,to analyze the correlation between its expression with clinical characteristics and prognosis,and to explore its molecular mechanism.Methods The transcriptome sequencing data of colon cancer and clinical data of the patients were downloaded from the TCGA database.The R4.1.2 software was used to extract the expression levels of FJX1 gene in colon cancer tissue and normal tissue,the Mann-Whitney U test was used to analyze the expression difference of FJX1 gene between the two groups,and the relationship between the FJX1 gene expression and clinical pathological characteristics of the patients was analyzed.The Kaplan-Meier method was utilized to analyze the correlation between the FJX1 gene expression and overall survival rate of the patients.The Cox regression analysis was utilized to analyze the molecular prognostic value of FJX1 gene in colon cancer.Moreover,the gene enrichment analysis was used to explore the action mechanism of FJX1 gene in colon cancer.Results Compared with normal tissues,the FJX1 gene expression level in colon cancer tissue was significantly up-regulated(P<0.001).The expression level of FJX1 gene had statistical difference among the patients with different Stage stages and M stages(P=0.038).The overall survival rate of the FJX1 gene high expression group was lower than that of the FJX1 gene low expression group(P<0.05).The multivariate Cox regression result showed that the FJX1 gene could serve as an independent prognostic factor for the patients with colon cancer(HR=1.133,95%CI:1.025-1.253,P=0.015).The gene enrichment results showed that FJX1 gene could exert its biological functions in vivo through cellular adhesion,cytokine interaction,ECM receptor interaction and cancer-related signaling pathways.Conclusion FJX1 gene is up-regulated in colon cancer tissue,which is correlated with the malignant progression characteristics of the patients and could serve as a potential molecular marker for colon cancer.
作者
薛丽
黄奔
XUE Li;HUANG Ben(Department of Laboratory Medicine,First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China)
出处
《国际检验医学杂志》
CAS
2022年第24期3043-3047,3052,共6页
International Journal of Laboratory Medicine
作者简介
薛丽,女,技师,主要从事临床检验研究;通信作者:黄奔,E-mail:HHHjoe123@163.com。
