摘要
建立了高效液相色谱法(HPLC)测定奶制品中苯甲酸、山梨酸、糖精钠、甲基香兰素和乙基香兰素的方法。样品经磷酸盐缓冲溶液和甲醇(V∶V=85∶15)直接提取,乙酸锌-亚铁氰化钾体系沉淀蛋白质,PXA固相萃取小柱净化,外标法和溶剂标准曲线定量。结果表明,5种食品添加剂在0.5~20.0μg·mL^(-1)浓度范围内呈良好线性,相关系数均大于0.9997,检出限均为1.00 mg·kg^(-1),定量限均为3.00 mg·kg^(-1)。3个不同添加浓度(5.0、10.0、50.0 mg·kg^(-1))的平均回收率在80.0%~100%之间,相对标准偏差(RSD,n=6)在0.40%~7.4%之间。
A method for the determination of benzoic acid,sorbic acid,saccharin sodium,methyl vanillin and ethyl vanillin in dairy products by High performance liquid chromatograph.The samples were directly extracted by phosphate buffer solution and methanol(V∶V=85∶15),protein precipitated by zinc acetate and potassium ferrocyanide system,purified by PXA solid phase extraction column,qualitative and quantitative by external standard method and solvent standard curve.The results showed that the five food additives showed good linearity in the concentration range of 0.5-20.0μg·mL^(-1),the correlation coefficients were greater than 0.9997,the limits of detection were1.00 mg·kg^(-1),and the limits of quantification were 3.00 mg·kg^(-1).The average recoveries of the three concentrations(5.0,10.0 and 50.0 mg·kg^(-1))ranged from 80.0%to 100%,and the relative standard deviations(RSD,n=6)ranged from 0.40%to 7.4%.
作者
张飞
杨涛
刘长勇
党富民
ZHANG Fei;YANG Tao;LIU Chang-yong;DANG Fu-min(Analysis and Testing Center,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2022年第11期2778-2782,共5页
Chemical Research and Application
基金
兵团农产品质量安全监管(葡萄储藏保鲜风险评估)项目资助
兵团农产品质量安全监管(标准制定)项目资助。
关键词
高效液相色谱仪
奶制品
固相萃取柱
食品添加剂
high performance liquid chromatograph
dairy products
solid phase extraction column
food additives
作者简介
联系人:党富民(1982-),男,高级实验师,主要从事食品安全检测与分析。E-mail:82862867@qq.com。
