摘要
目的探讨甲状腺功能正常的Graves病患者与健康对照者之间粪便肠道菌群结构及短链脂肪酸的差异,并明确促甲状腺素受体抗体(thyrotropin receptor auto-antibodies,TRAb)与差异菌群的相关性。方法选取30名治疗后甲状腺功能恢复正常的Graves病患者和32名健康对照者为研究对象,采用16S rRNA测序分析肠道菌群构成,气相色谱-质谱联用法(gas chromatography-mass spectrometry,GC-MS)方法测定短链脂肪酸浓度,分析TRAb抗体滴度与差异菌群的相关性。结果与对照组比较,Graves组患者的肠道菌群α多样性指数显著降低。在科水平,Graves组患者的拟杆菌(Bacteroidaceae)及瘤胃球菌(Ruminococcaceae)等相对丰度下降,普雷沃菌(Prevotellaceae)及韦荣球菌(Erysipelotrichaceae)丰度升高。在种水平,Graves组患者的霍氏真杆菌(Eubacterium_hallii)、瘤胃球菌及反刍动物真杆菌(Eubacterium-ventriosum)相对丰度升高,而瘤胃球菌(Ruminococcus-bicirculans)及普通拟杆菌(Bacteroide_vulgatus)等丰度下降,差异均有统计学意义。LEfSe分析显示,Graves组普雷沃菌(f_Prevotellaceae及g_unidentified_Prevotellaceae)及厌氧菌(s_Prevotella copri)富集。作为肠道菌群的重要代谢产物,Graves组患者的丙酸及丁酸浓度显著下降,丙酸浓度与瘤胃球菌丰度正相关。关联分析显示,血清TRAb抗体滴度与瘤胃球菌丰度呈负相关。结论即使甲状腺功能恢复正常,Graves病患者的肠道菌群及丙酸等浓度仍发生显著性改变,这一改变可能与血清TRAb抗体滴度相关。
Objective To investigate the differences in intestinal microbiota and short-chain fatty acids between patients suffering from Graves disease(GD)with normal thyroid function and healthy controls,and to analyze the correlation between thyrotropin receptor auto-antibodies(TRAb)and different intestinal microbiota.Methods Totally 30 Graves patients with normal thyroid function(GD group)and 32 healthy controls(Control)were enrolled in our study.Feces were collected,and 16S rRNA sequencing was used to analyze the structure of intestinal bacteria,and gas chromatography-mass spectrometry(GC-MS)was used to determine the content of short-chain fatty acids,the relationship between TRAb and intestinal microbiota was analyzed.Results Compared with the control group,α-diversity indexes of intestinal flora in GD patients were significantly decreased statistically.At the family level,the abundance of Bacteroidaceae and Ruminococcaceae decreased in GD patients,while the abundance of Prevotellaceae and Erysipelotrichaceae increased.At the species level,Eubacterium_hallii,Ruminococcus and Eubacterium_ventriosum were increased in GD patients,while Ruminococcus-bicirculans and Bacteroide_vulgatus decreased,and the differences were statistically significant.LEfSe analysis showed that Prevotellaceae(f_prevotellaceae and g_unidentified_prevotellaceae)and anaerobic bacteria(s_Prevotella copri)were enriched in GD group.The levels of propionic acid and butyric acid in GD patients were significantly decreased.The level of propionic acid was positively correlated with the abundance of Ruminococcaceae.Our analysis showed that serum TRAb antibody was inversely correlated with Ruminococcus abundance.Conclusion Even if the thyroid function of patients with Graves disease was normal,the intestinal flora and levels of propionic acid in patients were still significantly changed,and this change may be related to the serum TRAb antibody.
作者
王华
史婷婷
信中
华琳
李聪
曹秋梅
Wang Hua;Shi Tingting;Xin Zhong;Hua Lin;Li Cong;Cao Qiumei(Department of Emergency,Beijing Tongren Hospital,Capital Medical University,Beijing 102600,China;Department of Endocrinology,Beijing Tongren Hospital,Capital Medical University,Beijing 102600,China;Department of Mathematics,School of Biomedical Engineering,Capital Medical University,Beijing 100069,China)
出处
《首都医科大学学报》
CAS
北大核心
2022年第6期931-939,共9页
Journal of Capital Medical University
基金
北京市医管局“培育计划”项目(PX2021006)
首都医科大学校培育基金(PYZ21090)
首都医科大学附属北京同仁医院青年人才院内种子基金(2020-YJJ-ZZL-012)。
作者简介
Corresponding author:曹秋梅,E-mail:honeydoc@126.com。
