摘要
羧基化合物与植物的生长发育和胁迫应答密切相关。然而,由于羧基化合物结构的多样性、丰度差异大以及植物基质复杂等原因,对其高灵敏度、高覆盖度分析仍面临挑战。本研究利用稳定同位素标记辅助的超高效液相色谱-高分辨质谱联用技术(CIL-UPLC-MS),建立了水稻中羧基化合物的全分析方法。利用该方法在水稻中共发现了1 444个可能的羧基化合物,其中648个化合物得到了定性。同时,对镉胁迫下水稻根部和地上部分的羧基化合物进行了相对定量分析。结果表明,镉胁迫下,水稻根中的大部分羧基化合物含量显著降低,而地上部分中的绝大多数羧基化合物含量显著升高,表明水稻根部和地上部分在响应镉胁迫时,可能在羧酸代谢水平上采用了不同的应对策略。本研究提供了更多植物中的羧基化合物,为进一步揭示植物对镉胁迫的响应机制提供了更有效的分析方法和更可靠的科学数据。
Carboxylic compounds are closely related to plant growth and stress responses.The high sensitivity and high coverage analysis of carboxyl compounds is still a challenge due to the structural diversity, differences in abundance, and the complexity of plant substrates.In this study, we established a comprehensive profiling method of carboxylic compounds in rice by an integrated chemical isotope labeling combined with ultra-high performance liquid chromatography-high resolution mass spectrometry(CIL-UPLC-MS) analysis.Using this method, a total of 1 444 potential carboxylic compounds were detected in rice samples, of which 648 compounds could be qualitatively identified.In addition, we also performed a relative quantitative analysis of the carboxylic compounds in the root and shoot of rice under cadmium(Cd) stress.The results showed that under Cd stress, the contents of most carboxylic compounds in rice root were decreased significantly, while the contents of the majority of carboxyl compounds in shoot were increased significantly.The results indicated that rice root and shoot might adopt different coping strategies at the level of carboxylic acid metabolism in response to Cd stress.This study enriches the types of carboxylic compounds in plants, and provides a powerful tool for further revealing the response mechanism of plants to Cd stress.
作者
曾宸
蔡文静
叶甜甜
冯钰锜
ZENG Chen;CAI Wenjing;YE Tiantian;FENG Yuqi(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072)
出处
《分析科学学报》
CAS
CSCD
北大核心
2022年第5期529-537,共9页
Journal of Analytical Science
基金
国家自然科学基金(No.21635006,31670373,21721005)
武汉大学科研启动基金(No.413000129,413000162,413100098)。
关键词
羧基化合物
植物
镉胁迫
稳定同位素标记
液相色谱-质谱联用
Carboxylic compound
Plants
Cadmium stress
Isotope labeling
Ultra-high performance liquid chromatography-mass spectrometry
作者简介
通讯作者:叶甜甜,女,博士,副研究员,硕士研究生导师,研究方向:植物激素代谢路径的研究与色谱-质谱联用技术.E-mail:ye_tiantian@whu.edu.cn。
