摘要
目的:探讨黄芩素对咪喹莫特诱导的小鼠银屑病样皮损的影响及作用机制。方法:将60只BALB/c小鼠随机分为空白对照组、模型组、地塞米松组(5 mg/kg)、黄芩素低(15 mg/kg)、中(30 mg/kg)、高(60 mg/kg)剂量组,每组10只。外涂咪喹莫特诱导银屑病小鼠模型,同时灌胃给予不同剂量黄芩素,1次/d,连续7 d。银屑病皮损面积和疾病严重程度(PASI)评分动态观察小鼠皮损变化;HE染色观察皮损组织病理学改变;免疫组织化学法检测皮损组织微血管标志物CD31阳性表达并计算微血管密度(MVD);ELISA检测皮损组织IL-17、IL-23、TNF-α和IFN-γ含量;qRT-PCR和Western blot分别检测皮损组织TGF-β1、CTGF、VEGF mRNA及蛋白表达。结果:与空白对照组相比,模型组小鼠背部皮肤出现红斑、表皮肥厚、鳞屑成层、炎症浸润等现象,PASI评分、MVD值、皮损组织IL-17、IL-23、TNF-α、IFN-γ含量及TGF-β1、CTGF、VEGF mRNA和蛋白表达显著提高(P<0.05);与模型组相比,黄芩素中、高剂量组及地塞米松组小鼠皮肤红斑、鳞屑严重程度减轻,PASI评分、MVD值、皮损组织IL-17、IL-23、TNF-α、IFN-γ含量及TGF-β1、CTGF、VEGF mRNA和蛋白表达显著降低(P<0.05),而黄芩素低剂量组相关指标差异无统计学意义(P>0.05)。结论:黄芩素可减轻小鼠皮损组织炎症反应、抑制皮损组织血管新生,进而改善银屑病样皮损,可能通过阻断TGF-β1/CTGF信号通路发挥作用。
Objective:To investigate effect of baicalein on mouse psoriatic lesions induced by imiquimod and its mechanism.Methods:Sixty BALB/c mice were randomly divided into blank control group,model group,dexamethasone group(5 mg/kg),baicalein low(15 mg/kg),medium(30 mg/kg)and high(60 mg/kg)doses groups,with 10 mice in each group. Psoriasis mouse model was induced by imiquimod,and different doses of baicalein were administrated by gavage at the same time,once a day,for consecutive7 days. Changes of skin lesions in mice were dynamically observed by area of lesions and PASI score. Histopathological changes of skin were observed by HE staining. Immunohistochemical method was used to detect expression of microvascular positive marker CD31 and microvessel density(MVD)in skin lesions was calculated. IL-17,IL-23,TNF-α and IFN-γ levels in skin tissues were determined by ELISA. mRNA and protein expressions of TGF-β1,CTGF and VEGF in skin tissues were detected by qRT-PCR and Western blot.Results:Compared with blank control group,model group showed erythema,epidermal hypertrophy,scale formation,inflammatory infiltration and other phenomena on back skin of mice,PASI score,MVD value,contents of IL-17,IL-23,TNF-α,IFN-γ,mRNA and protein expressions of TGF-β1,CTGF,VEGF in skin tissue were significantly increased(P<0.05). Compared with model group,severity of skin erythema and scaly in baicalein medium and high doses groups and dexamethasone group were reduced,PASI score,MVD value,contents of IL-17,IL-23,TNF-α,IFN-γ,mRNA and protein expressions of TGF-β1,CTGF,VEGF in skin tissue were significantly decreased(P<0.05). However,there were no significant changes of related indexes in baicalein low-dose group(P>0.05).Conclusion:Baicalin can reduce inflammatory response and inhibit angiogenesis of skin tissue,thereby improving psoriatic lesions,which may act by blocking TGF-β1/CTGF signaling pathway.
作者
马晓惠
王增强
程晓蕾
毛婷婷
陈宏(指导)
MA Xiaohui;WANG Zengqiang;CHENG Xiaolei;MAO Tingting;CHEN Hong(Department of Dermatology,Tianjin Union Medical Center,Tianjin 300121,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2022年第17期2082-2086,共5页
Chinese Journal of Immunology
基金
天津市卫健委科技基金项目(2015KY29)。
作者简介
马晓惠,女,硕士,主治医师,主要从事皮肤科临床工作及银屑病、湿疹等相关基础研究,E-mail:maxiaohuimxh2008@163.com;通信作者:陈宏,女,博士,主任医师,主要从事银屑病、湿疹、系统性红斑狼疮等方面的研究,E-mail:baixue20042006@126.com。
