摘要
建立高效液相色谱法测定乳与乳制品中柚皮苷含量的方法。样品用甲醇提取,在284 nm波长下,流动相为体积分数0.1%醋酸水溶液-乙腈(75∶25,V/V),C_(18)色谱柱分离,经二极管阵列检测器检测,外标法定量。结果表明:柚皮苷在5~500μg/mL质量浓度范围内线性关系良好,相关系数为0.9999;分别向乳粉、液态乳和酸乳样品中在加入柚皮苷标准品0.02、0.04、0.08 g/100 g时,加标回收率分别为96.2%~103.2%、96.3%~103.7%和99.7%~104.1%,相对标准偏差分别为1.12%~2.31%、1.25%~1.85%和1.24%~1.66%。该检测方法简便、快速、准确、重复性好,能够测定乳与乳制品中柚皮苷的含量。
A high performance liquid chromatographic(HPLC)method was developed for the determination of naringin in milk and dairy products.Samples were extracted with methanol,and the analytes were separated on a C_(18) column using a mobile phase consisting of 0.1%acetic acid aqueous solution and acetonitrile(75:25,V/V),detected by a diode array detector at 284 nm and quantified by an external standard method.The results showed that the linearity of this method was good in the concentration range of 5–500μg/mL with a correlation coefficient of 0.9999.The recoveries of powdered and liquid milk power and yogurt spiked at 0.02,0.04,and 0.08 g/100 g were 96.2%–103.2%,96.3%–103.7%,and 99.7%–104.1%,respectively,with relative standard deviations(n=6)of 1.12%–2.31%,1.25%–1.85%,and 1.24%–1.66%,respectively.The reported method is simple,rapid,accurate,repeatable,and suitable for the determination of naringin in milk and dairy products.
作者
宫慧丽
段国霞
刘丽君
陈素敏
谢瑞龙
刘春霞
陈静
武伦玮
刘旺
唐烁
李翠枝
吕志勇
GONG Huili;DUAN Guoxia;LIU Lijun;CHEN Sumin;XIE Ruilong;LIU Chunxia;CHEN Jing;WU Lunwei;LIU Wang;TANG Shuo;LI Cuizhi;LÜZhiyong(Inner Mongolia Yili Industrial Group Co.Ltd.,Hohhot 010110,China)
出处
《乳业科学与技术》
2022年第5期27-31,共5页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
关键词
高效液相色谱法
乳制品
乳粉
液态乳
酸乳
柚皮苷
high performance liquid chromatography
dairy products
milk powder
liquid milk
yogurt
naringin
作者简介
第一作者:宫慧丽(1982-)(ORCID:0000-0002-6123-5167),女,工程师,硕士,研究方向为乳制品检测分析。E-mail:gonghuili@yili.com;通信作者:李翠枝(1969-)(ORCID:0000-0002-1997-7917),女,教授级高级工程师,硕士,研究方向为食品安全预防控制。E-mail:lczg@yili.com。
