摘要
目的建立本地区血小板库供者库,并基于以往献血频率、献血种类区分入库供者,探索提升血小板库应用效率的方法。方法1)依据献血频率和献血种类对本中心2011~2020年已加入中华骨髓库的献血者筛选归类,分别命名为储备供者、希望供者和活跃供者,按照活跃供者选择条件从2020年所有机采献血人群中收集这类供者,扩充本地区血小板库。2)2020年共筛选出符合活跃供者条件的献血者739例(含已入中华骨髓库活跃供者),收集其静脉血样5 mL,采用HLA高分辨基因分型方法对其进行HLA-A和-B位点检测、Q-PCR基因分型方法进行HPA检测。3)统计2021年本中心3类供者分别提供配型相合血小板的数量。结果1)构建了由以往不同捐献经历供者组成的太原地区血小板库,其中活跃供者739例、希望供者3840例、储备供者18715例。通过卡方检验,发现这3类供者ABO血型构成比无差异;性别均男性多于女性。2)2021年,加入血小板库的活跃供者再次捐献血小板的平均量与一般人群常规捐献之比为14.4∶3.98。3)2021年为PTR患者供应的142份相容性血小板中,83.8%来自活跃供者入库后的再次捐献,希望供者和储备供者作为有效补充分别提供了9.9%和6.3%。4)2021年本中心有HLA分型数据的库存机采血小板平均达到28.1%,54.2%的相容性血小板是从库存血小板产品中检索获得,及时满足了临床患者需要。结论整合资源、区分血小板库供者的活跃度可以降低建库成本,提高血小板库的应用效率。
Objective To establish the donor bank for local region and distinguish the donors based on the past blood donation frequency and blood donation type,so as to improve the application efficiency of platelet bank.Methods 1)According to the donation type and frequency of blood donors,the blood donors who had joined China Marrow Donor Program(CMDP)in our center from 2011 to 2020 were screened and classified.They are classified as reserve donors,prospective donors,and active donors.The donors,who met the selection conditions of active donors,were enrolled from all apheresis donors in 2020 to expand the local platelet bank.2)In 2020,739 blood donors who met the conditions of active donors were screened(including donors who had entered CMDP),and their HLA-A/B loci were detected by HLA high-resolution genotyping,and HPA was detected by Q-PCR genotyping.3)The compatible platelets provided by three types of donors in 2021 were calculated.Results 1)Taiyuan platelet bank,composed of donors with different previous donation experiences,had been constructed,including 739 active donors,3840 prospective donors and 18715 reserve donors.The composition ratio of ABO blood groups among three types of donors was found to be similar via chi square test;there were more male than female in three groups.2)In 2021,the ratio of the average redonation by active donors in the platelet bank to the regular donation by the general donors was 14.4∶3.98.3)Of the 142 compatible platelets,supplied to PTR patients in 2021,83.8%of them came from the redonation of active donors after registration in the bank,and 9.9%and 6.3%from prospective donors and reserve donors,respectively.4)In 2021,28.1%of the stored pheresis platalets in our center had HLA typing data,and 54.2%of compatible platelets were retrieved from the inventory,which timely met the needs of clinical patients.Conclusion Integrating resources and distinguishing the activity degree of donors in platelet bank can reduce the cost of bank building and improve the application efficiency of the platelet bank.
作者
张德梅
李卿
徐熠
梁雅珺
王菲
赵沛喆
白林
ZHANG Demei;LI Qing;XU Yi;LIANG Yajun;WANG Fei;ZHAO Peizhe;BAI Lin(Taiyuan Blood Center,Taiyuan 030024,China)
出处
《中国输血杂志》
CAS
2022年第9期970-973,共4页
Chinese Journal of Blood Transfusion
基金
山西省卫生健康委员会“四个一批”科技兴医创新计划项目:应用基因技术精准解决血小板输注无效的研究(2021XM13)。
作者简介
通信作者:白林(1963.04-),男,主任医师,主要从事血站业务及质量管理研究,电话:0351-8304025,Email:bangongshi8304005@163.com。
