摘要
目的探究糖尿病性视网膜病变(DR)相关标志物,分析循环miR-3197、miR-2116-5p在血糖控制达标的2型糖尿病视网膜病变患者中的表达及其临床价值。方法选取2018年7月~2020年3月在我院确诊为DR的2型糖尿病(T2DM)患者72例作为研究对象,并选择同期未有DR的T2DM患者72例作为对照组。对比两组患者的一般资料,通过μParaflo^(TM)MicroRNA微阵列法检测两组患者miRNA的表达水平,并采用逆转录定量PCR(RT-qPCR)技术进行验证。通过Spearman法和Pearson法分析DR患者血清miR-3197和miR-2116-5p水平与DR及各血液指标的相关性,并利用受试者工作特征曲线(receiver operating characteristic curve,ROC)分析循环miR-3197和miR-2116-5p单项检测以及两项联合检测诊断DR的敏感性和特异性,评估其诊断价值。结果DR组患者的GA、FPG、UACR以及HbA1c指标高于非DR组,差异具有统计学意义(P<0.05),两组患者在年龄、BMI、糖尿病病程、TC、Cr、HDL、LDL、TG以及BUN方面没有显著的组间差异(P>0.05)。μParaflo^(TM)MicroRNA微阵列检测到73种miRNA在DR病例中差异表达,其中miR-3197和miR-2116-5p在DR患者中的含量显著高于非DR患者,且与RT-qPCR分析验证结果一致。DR组患者血清miR-3197和miR-2116-5p水平分别与DR、GA、FPG、UACR以及HbA_(1c)呈正相关(均为P<0.001)。通过ROC曲线评估循环miR-3197和miR-2116-5p对DR的诊断敏感性和特异性,miR-3197和miR-2116-5p的AUC分别为0.903和0.875,miR-3197和miR-2116-5p联合检测的AUC为0.976,显著大于单项检测。结论循环miR-3197和miR-2116-5p在DR患者中显著上调,与DR病情严重程度及多种DR相关指标密切相关,可能参与疾病的发生发展,二者联合检测对DR有更高的诊断价值,提示miR-3197和miR-2116-5p联合检测可能在临床诊疗中具有一定的应用前景。
Objective To explore the related markers of diabetic retinopathy(DR)and analyze the expression and clinical value of circulating miR-3197 and miR-2116-5p in patients with type 2 diabetic retinopathy with blood glucose control.Methods 72 patients with type 2 diabetes(T2DM)who were diagnosed with diabetic retinopathy(DR)in our hospital from July 2018 to March 2020 were selected as the research objects,and 72 patients with T2DM who did not suffer from DR during the same period were selected as control group.To compare the general information of the two groups of patients,theμParaflo^(TM)MicroRNA microarray method was used to detect the miRNA expression levels of the two groups of patients,and verified by transcription quantitative PCR(RT-qPCR)technology.Spearman method and Pearson method were used to analyze the correlation between miR-3197 and miR-2116-5p in the serum of DR patients with DR and various biochemical indicators,and to analyze the circulating microRNAs by receiver operating characteristic curve(ROC).The diagnostic sensitivity and specificity of miR-3197 and miR-2116-5p single test and two combined tests for DR,to evaluate the diagnostic value.Results The GA,FPG,UACR,and HbA1c indicators of the DR group were higher than those of the non-DR group,and the difference was statistically significant(P<0.05).There was no significant difference between the groups in age,BMI,diabetes course,TC,Cr,HDL,LDL,TG and BUN(P>0.05).A total of 73 miRNAs were differentially expressed in DR cases throughμParaflo^(TM)MicroRNA microarray.Among them,the levels of miR-3197 and miR-2116-5p in DR patients were significantly higher than those in non-DR patients,and the results were verified by RT-qPCR analysis.The serum levels of miR-3197 and miR-2116-5p in the DR group were positively correlated with DR,GA,FPG,UACR,and HbA1c,respectively(all P<0.001).ROC curve analysis was used to evaluate circulating miR-3197 and miR-2116--5p.The AUCs of miR-3197 and miR-2116-5p were 0.903 and 0.875,respectively,and the AUC of miR-3197 and miR-2116-5p combined detection was 0.976,which was significantly greater than the single detection.Conclusion Circulating miR-3197 and miR-2116-5p are significantly up-regulated in DR patients,which are closely related to the severity of DR and a variety of DR related indicators,and may participate in the occurrence and development of DR.the combined detection of them has higher diagnostic value for DR and has a very promising application prospect in clinical treatment.
作者
张浩
方新梅
张玛丽
ZHANG Hao;FANG Xin-mei;ZHANG Ma-li(Department of Endocrinology,Baoding First Central Hospital,Baoding Hebei 071000)
出处
《临床输血与检验》
CAS
2022年第5期635-643,共9页
Journal of Clinical Transfusion and Laboratory Medicine
基金
保定市科技计划项目(No.2141ZF061)资助。
作者简介
张浩(1987-),女,河北保定人,主治医师,本科,主要从事内分泌与代谢病研究,(E-mail)shouji993197@163.com。
