摘要
为了提高能源微藻Chlorella vulgaris的固碳产油性能和进一步降低其培养过程培养成本,本研究在单因素结果的基础上,通过响应面法对其进行了优化;并在优化后的条件下,探讨了采用15%CO_(2)联合沼液替代传统培养基BG-11培养Chlorella vulgaris的可行性.结果表明,通气速率、光照强度、温度过高或过低都不利于小球藻生长、固碳和产油.响应面优化后得出当通气速率为0.075 m^(3)∙m^(-3)·min^(-1)、温度为28.5℃、光照强度为4950 lx时,小球藻的固碳和产油效果最佳,其生物质产率、平均固碳速率和油脂产率分别为0.20、0.367g·L^(-1)·d^(-1)和56.8 mg·L^(-1)·d^(-1).利用50%沼液联合15%CO_(2)培养Chlorella vulgaris时,其平均固碳速率、油脂产率分别为0.3304 g·L^(-1)·d^(-1)和42.81 mg·L^(-1)·d^(-1),此时沼液中氨氮、总磷、COD的利用率分别能够达到55.48%、41.95%和81.63%,沼液可有效替代BG-11培养基,可大幅度降低培养成本,实现废水和CO_(2)的资源化.
In order to improve the carbon fixation and lipid production performance of the microalgae Chlorella vulgaris and reduce its culture process and cultivation cost,in this study its culture process cultivation was further optimized by response surface methodology based on the single-factor results.And then,the feasibility of using 15%CO_(2)combined with biogas slurry to replace the traditional medium BG-11 for the cultivation of Chlorella vulgaris was investigated.The results showed that gas flow rate,light intensity and excessively high or low temperature were not conducive for the biomass growth,carbon sequestration and oil accumulation of Chlorella vulgaris.The maximal carbon sequestration and oil production were obtained under the optimized conditions of the gas flow rate 0.075 m^(3)∙m^(-3)·min^(-1),28.5℃with the light intensity of 4950 lx.The corresponding biomass yield,average carbon fixation rate and oil yield were 0.20 g·L^(-1)·d^(-1),0.367 g·L^(-1)·d^(-1)and 56.8 mg·L^(-1)·d^(-1)respectively.When Chlorella vulgaris was cultured with 50%digestate combined with 15%CO_(2),its average carbon fixation rate and oil yield were respectively 0.3304 g·L^(-1)·d^(-1)and 42.81 mg·L^(-1)·d^(-1),and the utilization rates of ammonia nitrogen,total phosphorus and COD in digestate were 55.48%,41.95%and 81.63%.The biogas slurry can be used to replace part of the BG-11 medium,which can greatly reduce the cultivation cost and realize the resource utilization of wastewater and CO_(2).
作者
杨豪
信欣
曹惜霜
刘洁
徐成华
刘建英
YANG Hao;XIN Xin;CAO Xishuang;LIU Jie;XU Chenghua;LIU Jianying(College of Resources and Environment,Chengdu University of Information Technology,Chengdu 610225;China-Serbia“the Belt and Road”Joint Laboratory on Environment and Energy,Chengdu 610225)
出处
《环境科学学报》
CAS
CSCD
北大核心
2022年第9期205-216,共12页
Acta Scientiae Circumstantiae
基金
四川省科技厅国际合作项目(No.2019YFH0133)
山西省气象局科研项目(No.SXKZDDW20217105)。
关键词
微藻
响应面
固碳
产油
沼液培养
microalgae
response surface
carbon fixation
lipid production
manure liquid digestate
作者简介
杨豪(1995—),男,E-mail:81027961@qq.com;责任作者:信欣,E-mail:178920302@qq.com。
