摘要
细菌耐药已成为21世纪影响全球公共卫生健康的热点问题,正严重影响临床治疗效果以及农业畜牧养殖业的发展。由于开发新型抗生素的速度远低于耐药细菌的进化速度,因此,增强传统抗生素的杀菌效果是解决细菌耐药这一复杂难题的重要途径。通过外源添加醇类与妥布霉素对大肠杆菌-BW25113进行杀灭测试,结果表明:筛选所得1,2-丁二醇能够显著增强氨基糖苷类抗生素对BW25113及临床耐药大肠杆菌的杀灭效果。通过妥布霉素摄取试验证实1,2-丁二醇能够促进大肠杆菌摄取妥布霉素到胞内,引起胞内抗生素增多进而导致细菌死亡,提示可由此实现其辅助杀菌的作用。
Bacterial drug resistance has become a hot issue affecting the global public health in the 21st century,which is seriously affecting the treatment effect and the development of agriculture and animal husbandry.Since the rate of developing new antibiotics is much slower than the evolutional speed of drug-resistant bacteria,improving the bactericidal effect of traditional antibiotics is an important way to solve the complex problem of bacterial resistance.The killing test against Escherichia coli BW25113 was carried out by adding the exogenous alcohols and tobramycin.The results showed that the 1,2-butanediol obtained from the screening could significantly enhance the killing effect of aminoglycoside antibiotics against BW25113 and the clinical drug-resistant Escherichia coli.The tobramycin uptake experiments have confirmed that 1,2-butanediol could promote the uptake of tobramycin into the cell by Escherichia coli,cause the increase of intracellular antibiotics and then lead to the bacterial death,suggesting that its auxiliary sterilization effect could be achieved.
作者
冯荆城
庄彬彬
李中燕
马月龙
李心想
陈雅娟
FENG Jing-cheng;ZHUANG Bin-bin;LI Zhong-yan;MA Yue-long;LI Xin-xiang;CHEN Ya-juan(Key Laboratory of Cellular Stress Response and Metabolic Regulation in Fujian Universities,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350108,China)
出处
《福建农业科技》
CAS
2022年第7期27-34,共8页
Fujian Agricultural Science and Technology
基金
福建省自然科学基金面上项目(2019J01278)。
作者简介
冯荆城,男,1997年生,硕士研究生,主要从事细菌耐药研究;通信作者:陈雅娟,女,1979年生,博士,讲师,主要从事细胞逆境响应与代谢调控研究(E-mail:cyj288@fjnu.edu.cn)。
