摘要
目的:探究地黄饮子对阿尔茨海默病(AD)模型小鼠海马组织氧化应激及炎性因子表达的影响。方法:3个月月龄SPF级PP/PS1双转基因雄性小鼠50只,随机分为安慰组(等体积的蒸馏水)、地黄饮子低剂量组(生药量为0.8 g/kg)、地黄饮子中剂量组(生药量为1.6 g/kg)、地黄饮子汤高剂量组(生药量为3.2 g/kg)、脑复康(吡拉西坦)组(0.63 g/kg),应用Morris水迷宫试验连续观察各组AD小鼠在第1周、第2周、第3周、第4周的认知功能变化。酶联免疫吸附试验(ELISA)检测炎性因子水平和氧化应激水平。结果:与安慰组比较,地黄饮子低、中、高剂量各组及脑复康组逃避潜伏期时间在治疗1、2、3、4 w后均逐渐缩短(P<0.05),且地黄饮子低、中、高剂量组呈剂量效应关系(P<0.05)。地黄饮子组高剂量组逃避潜伏期时间明显低于脑复康组(P<0.05),与安慰组相比,地黄饮子低、中、高剂量各组白细胞介素(IL)-1、IL-6、肿瘤坏死因子-α(TNF-α)以及超敏C-反应蛋白(hs-CRP)水平呈剂量效应的下降(P<0.05),且地黄饮子高剂量组明显低于脑复康组(P<0.05)。与安慰组相比,地黄饮子低、中、高剂量各组超氧化物歧化酶(SOD)、谷胱甘肽过氧化物(GSH-Px)和过氧化氢酶(CAT)水平呈剂量效应的增加(P<0.05),丙二醛(MDA)呈现剂量效应的降低(P<0.05),且地黄饮子高剂量组与脑复康组呈显著性差异(P<0.05)。结论:高剂量地黄饮子可改善AD模型小鼠的空间记忆能力,其机制可能与降低脑组织氧化应激水平及炎性因子表达有关。
Objective To investigate the effects of Dihuang Yinzi on the expression of oxidative stress and inflammatory factors in hippocampus of AD model mice.Method 50 3-month-old SPF PP/PS1 double transgenic male mice were selected. They were randomly divided into placebo group(equal volume of distilled water), DiHuang Yinzi low-dose group(0.8 g/kg crude drug), DiHuang Yinzi lmedium-dose group(1.6 g/kg crude drug), DiHuang Yinzi decoction high-dose group(3.2 g/kg crude drug), Naofukang(piracetan) group(0.63 g/kg), Morris water maze test was used to observe the cognitive function of AD mice in each group at week 1, 2, 3 and 4. The levels of inflammatory factors and oxidative stress were measured by ELISA.Results Compared with the placebo group, the incubation period of escape of DiHuang Yinzi low-dose, medium-dose and high-dose groups and cerebral rehabilitation group was gradually shortened after treatment 1, 2, 3 and 4 weeks(P<0.05), and there was a dose-effect relationship in dihuang Yinzi low-dose, medium-dose and high-dose groups(P<0.05). Compared with placebo group, Il-1, IL-6, TNF-α and hs-CRP levels in low-dose, medium-dose and high-dose groups of Rehmannia glutinosa decoction zi group showed dose-effect decrease(P<0.05). And the high dose of Dihuang Yinzi group was significantly lower than that of naofukang group(P<0.05). Compared with placebo group, the levels of SOD and GSH-Px in low-dose, medium-dose and high-dose groups showed a dose effect increase(P<0.05), while the levels of CAT and MDA showed a dose effect decrease(P<0.05), and there was a significant difference between high-dose group and Naofukang group(P<0.05).Conclusion High-dose dihuang Yinzi can improve the spatial memory ability of AD model mice, the mechanism may be related to the decrease of brain oxidative stress level and the expression of inflammatory factors.
作者
宋志勇
穆亚敏
SONG Zhi-yong;MU Ya-min(Xiangtan Medical and Health Vocational and Technical College,Xiangtan 411104,China)
出处
《吉林医学》
CAS
2022年第10期2597-2600,共4页
Jilin Medical Journal
基金
2021年第三批长株潭国家自主创新示范区建设专项科技创新培育项目[课题编号:CG-YB20211033]
湘潭医卫职业技术学院自科课题项目[课题编号:ZRKX2021-02]。
关键词
阿尔茨海默病
小鼠
地黄饮子
海马
氧化应激
炎性因子
Alzheimer disease
Mice
DiHuang Yinzi
Hippocampus
Oxidative stress
Inflammatory cytokines
