摘要
目的 探究髓系细胞触发受体1(TREM-1)对新西兰兔置入二代药物洗脱支架后支架内新生动脉粥样硬化(ISNA)及内膜覆盖的影响。方法 选择SPF级雄性新西兰兔20只,术中及术后4只兔死亡,其余16只均成功建立兔主动脉ISNA模型,按照随机数字表法分为实验组8只和对照组8只,高脂饮食喂养1个月后,造影下行腹主动脉球囊拉伤术及二代药物洗脱支架置入术。实验组术后给予TREM-1抑制剂LR12耳缘静脉注射,3次/周,对照组术后给予耳缘静脉注射生理盐水,3次/周。术后1个月调整饮食为1周高脂1周正常饮食交替喂养。3个月后于耳缘静脉采血,取血清行肝功能及血脂相关指标检查,同时行光学相干断层扫描(OCT)检查。结果 实验组TREM-1 mRNA和可溶性TREM-1水平明显低于对照组[0.53±0.27 vs 0.92±0.38,P=0.039;(75.83±13.78)pg/ml vs(110.50±39.20)pg/ml,P=0.043]。OCT分析显示,2组新生斑块长度、最小管腔面积、平均管腔面积、平均支架面积、最大斑块厚度、平均纤维帽厚度、最小纤维帽厚度、平均新生内膜厚度、未覆盖小梁、平均每截面小梁、平均新生内膜面积、未覆盖小梁截面和平均每枚支架截面比较,无统计学差异(P>0.05)。实验组平均斑块面积和平均斑块厚度明显低于对照组[(2.68±0.48)mm~2vs(4.25±1.33)mm~2,P=0.043;(376.23±60.85)μm vs(555.63±147.17)μm,P=0.041]。结论 TREM-1抑制剂可有效减少ISNA面积及厚度,有望成为预防ISNA的新型药物。
Objective To explore the effects of triggering receptor expressed on myeloid cells-1(TREM-1) on in-stent neoatherosclerosis(ISNA) and intimal coverage in New Zealand rabbits implanted with second-generation drug-eluting stent.Methods After 20 SPF-grade male New Zealand rabbits were fed with high-fat diet for 1 month, aortic balloon injury and second-generation drug-eluting stent implantation were performed under angiography.During and after operation, 4 rabbits died.The remaining 16 rabbits of successfully established ISNA model in the aortic stent were randomly divided into experimental group(n=8) and control group(n=8).The rabbits of the experimental group was given TREM-1 inhibitor LR12 for 3 times a week by ear vein, while those of the control group received same amount of normal saline.In 1 month after operation, the diet was adjusted to a week of high-fat diet and a week of normal diet alternately.After 3 months, the blood samples were harvested from the ear vein to test the levels of liver enzymes and blood lipids.Optical coherence tomography(OCT) was conducted at the same time.Results The levels of TREM-1 mRNA and soluble TREM-1 were significantly lower in the experimental group than the control group(0.53±0.27 vs 0.92±0.38,P=0.039;75.83±13.78 pg/ml vs 110.50±39.20 pg/ml, P=0.043).OCT scanning showed that there were no statistical differences between the two groups in plaque length, minimum lumen area, average lumen area, average stent area, maximum plaque thickness, average fiber cap thickness, minimum fiber cap thickness, average neointimal thickness, uncovered strut, average neointimal area, uncovered strut section and average stent section(P>0.05).The average plaque area was remarkably less and the average plaque thickness was thinner in the experimental group than the control group(2.68±0.48 mm~2 vs 4.25±1.33 mm~2,P=0.043;376.23±60.85 μm vs 555.63±147.17 μm, P=0.041).Conclusion TREM-1 inhibitor can effectively reduce the area and thickness of ISNA,so it is expected to be a new drug to prevent ISNA.
作者
蒋梦婷
张颖倩
方岩
袁晓航
韩燕
冯欢欢
高磊
Jiang Mengting;Zhang Yingqian;Fang Yan;Yuan Xiaohang;Han Yan;Feng Huanhuan;Gao Lei(Department of Cardiology,the Sixth Medical Center of Chinese PLA General Hospital,Beijing 100048,China)
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2022年第9期970-974,共5页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
国家自然科学基金(81970443)
北京市科技计划项目(Z171100001017158)。
作者简介
通信作者:高磊,Email:nkgaolei2010@126.com。
