摘要
目的分析RhD阴性孕产妇血小板抗体检测结果,探讨产前血小板抗体监测对新生儿同种免疫性血小板减少症的预防价值。方法采用固相凝集法对RhD阴性女性进行血小板抗体筛查,采用酶联免疫法对血小板抗体筛查阳性者进行血小板抗体特异性分析,并使用引物序列特异性聚合酶链式反应进行HPA基因分型。结果204例RhD阴性女性中血小板抗体阳性29例,阳性率为14.22%,其中抗-HLA抗体阳性20例(68.97%),抗-HPA抗体阳性4例(13.79%),抗-HLA类抗体和抗-HPA抗体均阳性者5例(17.24%)。其中1例血小板抗体阳性的孕妇其胎儿出生后出现免疫性血小板减少症状。结论对孕产妇进行血小板抗体筛查和鉴定,可为新生儿同种免疫性血小板减少症的预防和诊断提供依据。
Objective To analyze the platelet antibody specificity of RhD negative pregnant women and to investigate the correlation between platelet antibody and neonatal alloimmune thrombocytopenia(NAIT).Methods Platelet antibodies were detected by solid-phase agglutination in RhD negative women,then the platelet antibody specificity were identified by ELISA.HPA genotyping was performed by PCR-SSP.Results Among the 204 cases of RhD negative women,29 cases(14.22%)were positive of platelet antibodies,including 20 cases(68.97%)of anti-HLA positive,4 cases(13.79%)of anti-HPA positive,and 5 cases(17.24%)of anti-HLA and anti-HPA positive.One of the pregnant women with platelet antibody positive had a newborn diagnosed with NAIT.Conclusion The detection of platelet antibody in pregnant women can provide basis for the diagnosis and prevention of NAIT.
作者
阳智芬
旷开其
陈洁
谢毓滨
YANG Zhi-fen;KUANG Kai-qi;CHEN Jie;XIE Yu-bin(Institute of Blood Transfusion,Changsha Blood Center,Changsha 410001,Hunan,China;不详)
出处
《广东医学》
CAS
2022年第8期1001-1004,共4页
Guangdong Medical Journal
基金
湖南省卫生健康委科研计划课题项目(B20182028)。
作者简介
通信作者:谢毓滨,主任技师,E-mail:xieyubin7411@163.com。
