摘要
目的 研究高效液相色谱-串联质谱法(HPLC-MS/MS)测定动物源性食品中15种喹诺酮类化合物的基质效应及其补偿措施。方法 以鱼肉、猪肉和鸡肉作为典型基质,采用提取后加入法和动态多反应监测方式评价基质效应。结果 在三种基质中,60%以上化合物受到中等及强的基质效应,恩诺沙星、培氟沙星和达氟沙星受到强的基质增强效应;氟罗沙星受到强的抑制效应。前处理方法、基质种类、化合物种类以及质量浓度是影响喹诺酮类化合物基质效应的主要因素。结论 日常监测过程中,在不改变检测标准前处理的条件下,采取基质匹配标准曲线、同位素内标、稀释样品以及改善分离度并结合动态多反应监测方式能有效补偿喹诺酮类化合物的基质效应,提高其检测结果的可靠性和准确性。
Objective To study the matrix effects(MEs) and the compensation measures of 15 quinolones(QNs)determination in animal derived food by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods Fish,pork and chicken as typical substrates were tested by post-extraction spiking method and dynamic multiple reaction monitoring to evaluate matrix effects.Results More than 60% of the quinolones suffered moderate and strong matrix effects in the three samples. Enrofloxacin,pefloxacin and dalfloxacin had strongly matrix enhancement effect,and fleroxacin was strongly inhibited. Pretreatment methods,matrix species,compound species and mass concentration were the main factors affecting the matrix effect of quinolones.Conclusion In the process of daily monitoring which the pretreatment method specified in the test standard cannot be changed casually,matrix matching standard curve,isotope internal standard,diluted sample and improving chromatography resolution combined with dynamic multi reaction monitoring could effectively compensate the matrix effect of quinolones and improve the reliability and accuracy of the detection results.
作者
李道霞
黄丽娟
辜慧
蒋燕舒
余晓琴
李澍才
LI Daoxia;HUAGN Lijuan;GU Hui;JIANG Yanshu;YU Xiaoqin;LI Shucai(Sichuan Institute of Food Inspection,Sichuan Chengdu 610097,China;Key Laboratory of Baijiu Supervising Technology for State Market Regulation,Sichuan Chengdu 610097,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2022年第4期693-699,共7页
Chinese Journal of Food Hygiene
基金
国家重点研发计划项目(2017YFC1601300)。
关键词
喹诺酮类
基质效应
动态多反应监测
基质效应评价
高效液相色谱-串联质谱法
基质效应补偿
Quinolones
matrix effects
dynamic multi reaction monitoring
matrix effect evaluation
high performance liquid chromatography-tandem mass spectrometry
matrix effect compensation
作者简介
李道霞,女,主管药师研究方向为食品安全检测与标准研究,E-mail:398090516@qq.com;通信作者:李澍才,男,主管药师研究方向为食品理化检验与标准研究,E-mail:506814997@qq.com。
