摘要
目的探讨慢性亚砷酸钠染毒对大鼠肝损伤的作用。方法选择健康成年SD大鼠56只,雌雄各半,随机纳入对照组、低暴露组、中暴露组和高暴露组。暴露组通过自由饮用亚砷酸钠水溶液染毒,剂量分别为2、10和50 mg/L,连续染毒24周,对照组给予去离子水。测量大鼠体重和肝重,计算肝系数;采用原子荧光分光光度法检测尿砷含量;肝组织切片采用醋酸铀和柠檬酸铅双重染色,电镜下观察肝组织形态。结果各组雄性、雌性大鼠体重随染毒时间呈上升趋势(Waldχ^(2)=3610.621,P<0.001;Waldχ^(2)=2186.217,P<0.001),24周后各组雄性、雌性大鼠体重变化差异明显,时间与组间存在交互作用(Waldχ^(2)=15.874,P=0.001;Waldχ^(2)=9.460,P=0.024)。各组大鼠肝重和肝系数差异均有统计学意义(雄性:F=18.964、29.968,均P<0.001;雌性:F=11.919、15.070,均P<0.001),高暴露组雄性大鼠肝重和肝系数最低,分别为(10.17±1.15)g和(1.99±0.21)%;高暴露组雌性大鼠肝重和肝系数最高,分别为(12.91±1.29)g和(4.10±0.56)%。对照组、低暴露组、中暴露组和高暴露组大鼠尿砷含量[M(Q_(R))]分别为25.60(30.27)、146.56(101.06)、1034.68(600.06)和3796.98(19966.89)μg/L,差异有统计学意义(χ^(2)=50.211,P<0.001);中、高暴露组大鼠尿砷含量高于对照组(均P<0.001)。低、中暴露组大鼠可见肝细胞水肿;高暴露组大鼠可见肝细胞水肿、灶性坏死,毛细胆管增生和毛细胆管旁细胞质溶解。结论慢性砷暴露可导致大鼠肝组织形态变化,且随着暴露剂量增加,肝损伤加重。
Objective To examine the effect of chronic exposure to sodium arsenite on liver damages in rats.Methods Fifty-six healthy adult SD rats(28 males and 28 females)were randomly divided into 4 groups.Rats in the low-,medium-and high-dose groups were given sodium arsenite solutions at doses of 2,10 and 50 mg/L for successive 24 weeks,while animals in the control group were given deionized water.The rat body and liver weights were measured and the liver coefficient was estimated.The urine arsenic level was detected using atomic fluorescence spectrometry,and hepatic tissue sections were stained with uranium acetate and lead citrate for morphological observations under an electron microscope.Results The body weights of both male and female rats appeared a tendency towards a rise with the duration of exposure to sodium arsenite(male rat:Waldχ^(2)=3610.621,P<0.001;female rat:Waldχ^(2)=2186.217,P<0.001,and there were no significant differences in the rat body weight 24 weeks post-exposure to sodium arsenite in each group,while there was an interaction between time and group(male rat:Waldχ^(2)=15.874,P=0.001;Waldχ^(2)=9.460,P=0.024).There were significant differences in the rat liver weight and liver coefficient in each group(male rat:F=18.964 and 29.968,both P<0.001;female rat:F=11.919 and 15.070,both P<0.001),with the lowest liver weight(10.17±1.15)g and liver coefficient(1.99±0.21)%measured in male rats in the high-dose group,and the highest liver weight(12.91±1.29)g and liver coefficient(4.10±0.56)%in female rats in the high-dose group.The median urine arsenic levels(interquartile range)were 25.60(30.27),146.56(101.06),1034.68(600.06)and 3796.98(19966.89)μg/L in rats in the control,low-dose,medium-dose and high-dose groups,respectively(χ^(2)=50.211,P<0.001),and the urine arsenic level was significantly higher in the medium-and high-dose groups than in the control group(both P<0.001).Hepatic edema was seen in rats in the low-and medium-dose groups,and hepatic edema,focal hepatic cell necrosis,hyperplasia of bile capillaries and peri-bile capillary endolysis were observed in rats in the high-dose group.Conclusions Chronic exposure to arsenic may cause morphological alterations of rat hepatic tissues,and the rat hepatic damage aggravates with the dose of exposure to arsenic.
作者
丁关鑫
黄佳
林勤
夏荣香
吴顺华
张玲
DING Guanxin;HUANG Jia;LIN Qin;XIA Rongxiang;WU Shunhua;ZHANG Ling(School of Public Health,Xinjiang Medical University,Urumqi,Xinjiang 830011,China;Xinjiang Center for Disease Control and Prevention,Urumqi,Xinjiang 830002,China)
出处
《预防医学》
2022年第9期887-892,共6页
CHINA PREVENTIVE MEDICINE JOURNAL
基金
国家自然科学基金项目(81560520)。
关键词
砷暴露
亚砷酸钠
肝损伤
尿砷
arsenic exposure
sodium arsenite
liver damage
urine arsenic
作者简介
丁关鑫,硕士研究生在读;通信作者:张玲,E-mail:549307703@qq.com。
