摘要
为鉴别及检测不同地区市售羊乳粉的品质,基于反相高效液相色谱(reversed-phase high performance liquid chromatography,RP-HPLC)技术,将35种市售羊乳粉根据陕西地区和非陕西地区、纯羊乳粉和配方羊乳粉进行分类与检测,研究不同产地、不同种类羊乳粉的差异,对羊乳粉品质进行分析判别。结果表明:对市售羊乳粉乳蛋白进行RP-HPLC测定发现,配方羊乳粉α_(s2)-酪蛋白(α_(s2)-casein,α_(s2)-CN)和β-CN相对含量分别低于纯羊乳粉20%和24%,κ-CN和乳清蛋白相对含量分别高于纯羊乳粉30%和50%;不同地区纯羊乳粉乳蛋白组成及含量相似。因此,通过RP-HPLC可以实现对市售羊乳粉中乳蛋白的测定和质量评价。
To identify and test the quality of commercial goat milk powder sold in different areas,reversed-phase high performance liquid chromatography(RP-HPLC)was used to determine the protein composition of 35 samples of pure and formulated goat milk powder purchased in different regions(Shaanxi and non-Shaanxi)of China and in other countries,and the difference in protein composition among goat milk powder from different production areas and that between pure and formulated goat milk powder were analyzed.The results showed that the contents ofα_(s2)-casein andβ-casein in formulated milk powder were 20%and 24%lower than those in pure milk powder,respectively,and the contents ofκ-casein and whey proteins were 30%and 50%higher than those in pure milk powder,respectively.However,the protein composition and content of pure goat milk powder from different regions were similar.Therefore,RP-HPLC is useful for protein quality evaluation of commercial goat milk powder.
作者
宋卓岩
乔春艳
张雪茹
郝果
刘永峰
SONG Zhuoyan;QIAO Chunyan;ZHANG Xueru;HAO Guo;LIU Yongfeng(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an 710062,China;Fuping County Testing and Inspection Center,Fuping 711700,China)
出处
《乳业科学与技术》
2022年第4期52-56,共5页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
陕西省重点研发计划项目(2022ZDLNY04-09)
咸阳市科技项目(2021ZDZX-NY-0014)
国家市场监督管理总局科技计划项目(2021MK106)。
关键词
反向高效液相色谱
乳清蛋白
酪蛋白
羊乳粉
鉴定
reversed-phase high performance liquid chromatography
whey protein
casein
goat milk powder
identification
作者简介
第一作者:宋卓岩(1999-)(ORCID:0000-0003-1076-5413),男,硕士研究生,研究方向为乳品科学。E-mail:547616346@qq.com;通信作者:刘永峰(1981-)(ORCID:0000-0003-2563-0407),男,教授,博士,研究方向为畜产品科学。E-mail:yongfeng200@126.com。
