摘要
在鸡源大肠杆菌临床菌株E41中,构建双组分系统cpxR基因缺失株及回补株,并研究其对相关生物学特性的影响。采用Red同源重组的方法构建cpxR基因缺失株,并通过pBAD/HisA质粒构建回补株。比较临床野生株、缺失株和回补株生长曲线、运动能力、生物被膜形成能力、稳定性、药物敏感性等差异。成功构建缺失株ΔcpxR和回补株CΔcpxR。与野生株E41相比,ΔcpxR缺失株在生长曲线、运动能力及生物被膜形成方面影响不显著。在缺失株稳定性测定中,缺失株在传代15 d后,仍具有良好的稳定性。在药物敏感性测定中,cpxR的缺失导致菌株对链霉素和多西环素的MIC升高2倍,对氟苯尼考的MIC降低至原来的1/2。综上,双组分系统cpxR基因缺失对细菌药物敏感性有明显影响,对其他方面的作用影响不显著,其功能可能被另外的因素代偿性补偿。
The two-component system of cpxR gene deletion strain and complemented strain in chicken-derived Escherichia coli clinical strain E41 were constructed, and their effects on related biological properties were studied. The Red homologous recombination method was used to construct the cpxR gene deletion strain and the complemented strain of the pBAD/HisA plasmid. The differences in growth curve, motility, biofilm formation ability, stability of the deletion strain and sensitivity to antibacterial agents were compared among the clinical wild strain, deletion strain and complemented strain. The deletion strain ΔcpxR and the complemented strain CΔcpxR were successfully constructed. Compared with the clinical wild strain E41, ΔcpxR strain had no significant difference on growth curve, motility and biofilm formation ability. In the deletion strain stability assay,the deletion strain remained stable after 15 d of passage. In the antimicrobial susceptibility test, ΔcpxR strain resulted in the two-fold increase in MIC to streptomycin and doxycycline, and a one-half decrease in MIC to florfenicol. These results indicated that the deletion of cpxR gene in the two-component system had a significant effect on the drug sensitivity of bacteria, while the effect of other aspects was not significant, and its function could be compensated by other factors.
作者
刘志欢
宋雪艳
谢翠萍
陶梦珂
张鲁星
李苗苗
刘建华
LIU Zhi-huan;SONG Xue-yan;XIE Cui-ping;TAO Meng-ke;ZHANG Lu-xing;LI Miao-miao;LIU Jian-hua(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China)
出处
《江西农业学报》
CAS
2022年第6期98-103,共6页
Acta Agriculturae Jiangxi
基金
国家自然科学基金资助项目(32072914)。
作者简介
刘志欢(1995-),男,河南信阳人,硕士研究生,主要从事细菌耐药性工作;通信作者:刘建华。
