摘要
为探讨蚯蚓对不同类型土壤-植物系统中抗生素及其抗生素抗性基因(ARGs)的消减规律,文章通过盆栽实验种植草本类马唐与叶菜类上海青,采用高效液相色谱质谱联和实时荧光定量PCR技术从根际土、植物叶际和植物根部3个不同介质分析磺胺类抗生素、四环素类抗生素、喹诺酮类抗生素的含量与磺胺类抗性基因、四环素类抗性基因、喹诺酮类抗性基因的丰度。研究表明,蚯蚓可以有效降低根际土、植物叶际和植物根部的抗生素含量,分别降低5.55%~8.31%、5.89%~13.36%、18.76%~45.6%;蚯蚓可显著降低根际土壤、植物叶际、根内生菌的ARGs丰度,分别降低34.71%~64.97%、53.68%~60.13%、18.99%~31.51%。结果表明,蚯蚓对不同介质ARGs影响有所差异,对于根际土壤,蚯蚓对磺胺类抗性基因sul2的影响最为显著;对于叶际部分,蚯蚓对四环素类抗性基因tetM的去除率最为显著;而对于根内生菌,蚯蚓对草本类马塘中的tetM去除率最高,但对叶菜类上海青中的sul2去除率却为最高。通过热图分析,tetM在土壤-植物系统中丰度分布最为稳定,而四环素类抗性基因tetG在植物体内中的丰度分布显著高于根际土壤。
In order to explore the law of ARGs(santibiotic and antibiotic resistance genes) elimination by earthworm in different soil-plant systems, pot experiments were carried out, for which herbaceous Digitaria sanguinalis and Chinese cabbage(Shanghai greens) were planted, and the instruments of HPLC/MS/MS and real-time fluorescent quantitative PCR technology were used to analyze the content of sulfonamide antibiotics(SAs), tetracycline antibiotics(TCs), quinolone antibiotics(QNs) which were contained in three different media: rhizosphere soil, phyllosphere and plant roots, as well as the abundance of sulfa resistance genes, tetracycline resistance genes, quinolone resistance genes. The results showed that earthworms could effectively reduce the antibiotic content in rhizosphere soil, phyllosphere and plant roots by 5.55%~8.31%,5.89%~13.36% and 18.76%~45.6%, respectively. Earthworms remarkably reduced the ARGs abundance of rhizosphere soil,phyllosphere and root endophytic bacteria by 34.71%~64.97%, 53.68%~60.13% and 18.99%~31.51%, respectively. Findings also were that the effects of earthworms on ARGs varied in different media, for instance, as to rhizosphere soil, earthworms’ effect on sul2 resistance gene was fairly great, as to phyllosphere, the removal rate of tetM resistance gene was rather high, and for the endophytic bacteria, the removal rate of tetM from herbaceous Digitaria sanguinalis was the highest, while the removal rate of sul2 from leaf vegetable(Shanghai green) was the highest. The abundance distribution of tetM in soil-plant system was most stable, and the abundance distribution of tetG in plant was significantly higher than that in rhizosphere soil.
作者
何文程
陈明龙
史鑫成
包灿鑫
张园
HE Wencheng;CHEN Minglong;SHI Xincheng;BAO Canxin;ZHANG Yuan(School of Environment and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2022年第5期92-98,共7页
Environmental Science & Technology
基金
国家自然科学基金项目(41701564)
苏州市科技计划项目(SNG201613)
江苏省高校自然科学基金项目(17KJB610010)。
关键词
土壤-植物系统
抗生素
抗性基因
蚯蚓
soil-plant system
antibiotics
antibiotic resistance genes
earthworm
作者简介
何文程(1997-),男,硕士,主要从事土壤污染与治理研究,(电子信箱)827496854@qq.com;通讯作者:张园,女.副教授,博士,研究方向为土壤污染与治理修复,(电子信箱)yuanzhang_1001@mail.usts.edu.cn。
