摘要
目的:探讨脓毒症患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中长链非编码RNA TMEVPG1变化及临床意义。方法:选取2021年1月至12月江苏大学附属人民医院重症医学科收治的31例脓毒症患者为脓毒症组,另选取同时期来院体检健康者32例为对照组,收集外周血,分离PBMCs,实时荧光定量PCR检测TMEVPG1和干扰素(interferon,IFN)-γmRNA水平;相关性分析TMEVPG1与IFN-γmRNA、APACHEⅡ评分、外周血中性粒细胞计数/淋巴细胞计数比值(neutrophil to lymphocyte ratio,NLR)、外周血中性粒细胞计数/淋巴细胞和血小板计数比值(neutrophil to lymphocyte and platelet ratio,N/LPR)的关系;ROC曲线分析TMEVPG1对脓毒症的诊断价值。结果:脓毒症组PBMCs中TMEVPG1水平明显低于对照组(P<0.001),相关性分析结果显示,脓毒症患者PBMCs中TMEVPG1与APACHEⅡ评分、NLR、N/LPR之间均呈显著负相关;与对照组相比,脓毒症组PBMCs中IFN-γmRNA表达下调(P<0.001),且与TMEVPG1水平存在明显正相关。ROC曲线分析显示,TMEVPG1能够将脓毒症患者和健康对照者区分开。结论:TMEVPG1在脓毒症患者外周血中显著降低,可能通过调控IFN-γ的表达参与脓毒症的发生发展过程。
Objective:To investigate the expression of long noncoding RNA TMEVPG1 in peripheral blood mononuclear cells(PBMCs)from sepsis patients and the corresponding clinical significance.Methods:A total of 31 sepsis patients admitted to the Department of Critical Care Medicine of the Affiliated People′s Hospital of Jiangsu University from January 2021 to December 2021 were selected as the sepsis group,and 32 healthy subjects as the control group.The transcript levels of TMEVPG1 and interferon-γ(IFN-γ)in PBMCs were detected by real-time quantitative PCR.The relationship between TMEVPG1 levels and IFN-γmRNA levels,APACHEⅡscore,neutrophil to lymphocyte ratio(NLR),and neutrophil to lymphocyte and platelet ratio(N/LPR)were analyzed.ROC curve was performed to analyze the diagnostic value of TMEVPG1 for sepsis.Results:The transcript levels of TMEVPG1 in PBMCs from sepsis patients were significantly decreased compared with the controls.There was a significantly inverse correlation between TMEVPG1 levels and APACHEⅡscore of sepsis patients.Meanwhile,TMEVPG1 expression also negatively correlated with NLR and N/LPR in patients with sepsis.Compared with the controls,IFN-γmRNA expression in PBMCs from sepsis patients was downregulated,and there was a significantly positive correlation between TMEVPG1 levels and IFN-γmRNA levels in sepsis patients.ROC curves showed that TMEVPG1 could distinguish sepsis patients from healthy controls.Conclusion:Our findings indicated that TMEVPG1 was decreased in the peripheral blood of sepsis patients and might play a pivotal role in the pathogenesis of sepsis by regulating the expression of IFN-γ.
作者
徐娟
彭辉勇
蔡燕
XU Juan;PENG Huiyong;CAI Yan(Department of Critical Care Medicine,Affiliated People's Hospital of Jiangsu University,Zhenjiang Jiangsu 212002,China;Department of Clinical Laboratory,Affiliated People's Hospital of Jiangsu University,Zhenjiang Jiangsu 212002,China)
出处
《江苏大学学报（医学版）》
CAS
2022年第5期422-426,432,共6页
Journal of Jiangsu University：Medicine Edition
基金
镇江市社会发展项目(SH2021059)
江苏省青年医学重点人才项目(QNRC2016455)。
作者简介
徐娟(1988-),女,硕士研究生;通讯作者:蔡燕,副主任医师,E-mail:caiyan1980117@126.com。
