摘要
目的:探讨Kruppel样因子17(Kruppel-like factor 17,KLF17)、转录辅助因子退变样蛋白4(vestigial like family member 4,VGLL4)在子宫内膜样腺癌组织中的表达,了解二者在不同病理类型的表达差异及其与临床病理特征之间的联系。方法:选择2015年6月-2020年4月清远市妇幼保健院妇产科收集经病理确诊的标本共91例,子宫内膜样腺癌组织(41例),子宫内膜单纯性增生组织(28例),正常子宫内膜组织(22例)。运用免疫组化法检测KLF17、VGLL4的表达水平,分析KLF17、VGLL4与临床病理特征的关系。结果:KLF17在子宫内膜样腺癌及子宫内膜单纯性增生的阳性表达率分别为80.5%、25.0%,高于正常子宫内膜的18.2%,差异有统计学意义(P<0.05)。VGLL4在子宫内膜样腺癌的阳性表达率24.4%,低于子宫内膜单纯性增生、正常子宫内膜的71.4%、77.3%,差异有统计学意义(P<0.05)。KLF17与VGLL4在子宫内膜样腺癌中的表达,二者之间存在负相关(r=-0.437,P<0.05)。在人子宫内膜样腺癌组织中提示VGLL4表达与子宫内膜样腺癌分化程度存在正相关(r=0.346,P<0.05)。KLF17在子宫内膜样腺癌中的表达与患者年龄、FIGO分期、肌层浸润深度、分化程度、淋巴结转移无关(P>0.05)。VGLL4在子宫内膜样腺癌中的表达与患者年龄、FIGO分期、肌层浸润深度及淋巴结转移等无关(P>0.05),而与分化程度有相关性,分化程度越高,表达越强(P<0.05)。结论:在子宫内膜样腺癌组织中,KLF17表达上调和VGLL4表达下调,二者可能与内膜病变的发生发展有关。
Objective:To explore the expression levels of Kruppel-like factor 17(KLF17)and vestigial like family member 4(VGLL4)in endometrioid adenocarcinoma,and to understand their different pathological types,meanwhile,the expression difference and its relationship with clinicopathological characteristics.Method:From June 2015 to April 2020,91 cases of pathologically confirmed specimens in the Obstetrics and Gynecology Department of Qingyuan Maternal and Child Health Hospital were collected,including endometrioid adenocarcinoma tissue(41 cases),simple endometrial hyperplasia tissue(28 cases),and normal endometrial tissue(22 cases).Then,immunohistochemistry was used to test the expression level of KLF17 and VGLL4,and the relationship between KLF17,VGLL4 and clinicopathological characteristics was analyzed.Result:The positive expression rates of KLF17 in endometrioid adenocarcinoma and endometrial simple hyperplasia were 80.5%and 25.0%,respectively,higher than 18.2%in normal endometrium,and the difference was statistically significant(P<0.05).The positive expression rate of VGLL4 in endometrioid adenocarcinoma was 24.4%,which was lower than 71.4%and 77.3%of simple endometrial hyperplasia and normal endometrium,and the difference was statistically significant(P<0.05).There was a negative correlation between the expressions of KLF17 and VGLL4 in endometrioid adenocarcinoma(r=-0.437,P<0.05).The expression of VGLL4 in human endometrioid adenocarcinoma was positively correlated with the degree of differentiation of endometrioid adenocarcinoma(r=0.346,P<0.05).The expression of KLF17 in endometrioid adenocarcinoma was not associated with patient age,FIGO stage,depth of myometrial invasion,degree of differentiation and lymph node metastasis(P>0.05).The expression of VGLL4 in endometrioid adenocarcinoma was not related to patient age,FIGO stage,depth of myometrial invasion,lymph node metastasis(P>0.05).There was a correlation with the degree of differentiation,the higher the degree of differentiation,the stronger the expression(P<0.05).Conclusion:In endometrioid adenocarcinoma tissue,the expression of KLF17 is up-regulated and the expression of VGLL4 is down-regulated,both of which may be related to the occurrence and development of endometrial lesions.
作者
陈海红
胡庆兰
蒋学风
罗志峰
刘海红
CHEN Haihong;HU Qinglan;JIANG Xuefeng;LUO Zhifeng;LIU Haihong(Qingyuan Maternal and Child Health Hospital,Qingyuan 511500,China;不详)
出处
《中外医学研究》
2022年第20期13-17,共5页
CHINESE AND FOREIGN MEDICAL RESEARCH
